Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 6, 2025

Measuring Ascending Aortic Stiffness In Vivo in Mice Using Ultrasound

Measuring Ascending Aortic Stiffness In Vivo in Mice Using Ultrasound

Published on: December 2, 2014

Exploring aortic stiffness in aging mice: a comprehensive methodological overview.

Laetitia Vanalderwiert¹, Auberi Henry¹, Juliana Martins de Souza E Silva²

¹UMR CNRS 7369 MEDyC, University of Reims Champagne-Ardenne, Reims 51100, France.

|December 3, 2024

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Exploring mechanisms of scar-free skin wound healing in adult zebrafish in comparison to mouse.

PLoS genetics·2026

Same author

Characterization and development of a gelatin/elastin methacrylamide-based bioink for creating a 3D bioprinted human skin model.

International journal of biological macromolecules·2026

Same author

Outcomes and Validation of Histopathological Scores in Pediatric ANCA-vasculitis.

Kidney international reports·2026

Same author

Systemic clinical-grade recombinant decorin reduces disease burden and fibrosis in advanced recessive dystrophic epidermolysis bullosa.

Molecular therapy. Advances·2026

Same author

Non-clinical pharmacology, pharmacokinetics, and safety evaluation of TAX2, a first-in-class peptide targeting the TSP-1/CD47 matricellular axis.

Toxicology and applied pharmacology·2026

Same author

Lymphoid-Tissue-on-Chip Recapitulates Human Antibody Responses In Vitro.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Summary

Vascular aging causes aortic stiffness, a precursor to hypertension and other diseases. A new multi-scale method using decision trees effectively assesses aortic stiffness in preclinical models, revealing cellular and matrix changes.

Area of Science:

Cardiovascular Research
Aging Biology
Biomedical Engineering

Background:

Vascular stiffening is an early sign of aging, linked to hypertension, renal failure, and Alzheimer's disease.
Current diagnostic methods for vascular stiffness are often impractical for widespread use or unfamiliar to clinicians.
Preclinical stiffness studies are frequently incomplete, hindering a full understanding of aging-related vascular changes.

Purpose of the Study:

To propose and validate a systematic, multi-scale, and multimodal method for evaluating aortic stiffness using decision trees.
To enhance the understanding of aging disease progression through comprehensive aortic stiffness assessment.
To encourage the development and adoption of clinical tools for vascular function assessment.

Main Methods:

Keywords:

ageing aortic stiffness collagen fibers elastic fibers extracellular matrix remodeling methodological process

More Related Videos

Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy

Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy

Published on: October 19, 2016

Echocardiographic Approaches and Protocols for Comprehensive Phenotypic Characterization of Valvular Heart Disease in Mice

Echocardiographic Approaches and Protocols for Comprehensive Phenotypic Characterization of Valvular Heart Disease in Mice

Published on: February 14, 2017

Related Experiment Videos

Last Updated: Jun 6, 2025

Measuring Ascending Aortic Stiffness In Vivo in Mice Using Ultrasound

Measuring Ascending Aortic Stiffness In Vivo in Mice Using Ultrasound

Published on: December 2, 2014

Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy

Measuring the Stiffness of Ex Vivo Mouse Aortas Using Atomic Force Microscopy

Published on: October 19, 2016

Echocardiographic Approaches and Protocols for Comprehensive Phenotypic Characterization of Valvular Heart Disease in Mice

Echocardiographic Approaches and Protocols for Comprehensive Phenotypic Characterization of Valvular Heart Disease in Mice

Published on: February 14, 2017

Development of a systematic method employing decision trees for multi-scale and multimodal analysis.
Application of the method to analyze aortic tissue from old and young mice.
Evaluation of cellular function (endothelial and smooth muscle cells) and extracellular matrix remodeling.

Main Results:

The proposed method successfully identified functional alterations in endothelial and smooth muscle cells, favoring constriction.
Significant extracellular matrix remodeling was observed, including elastic fiber degradation and collagen accumulation in aged aortas.
These changes collectively contribute to vascular rigidity, a precursor to arterial hypertension.

Conclusions:

The developed systematic method improves preclinical understanding of aortic stiffness and aging.
Findings highlight the critical role of cellular and matrix changes in driving vascular rigidity.
The study advocates for clinical tools to assess vascular function, crucial for preventing age-related pathologies.