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Updated: Apr 16, 2026
Fluorimetric Techniques for the Assessment of Sperm Membranes
Published on: November 28, 2018
The correlation between abnormal Krüger strict morphology and the sperm DNA fragmentation index
Tamyres Souza Garcia Alvim Ranzato1, Mariana Duque de Mello2, Paula Fontoura Coelho de Souza2
1Fluminense Federal University Niterói, RJ, Brazil.
Objective:
This study aims to investigate whether alterations in sperm DNA fragmentation rates are more frequent when Krüger strict morphology is ≥4% (normal).
Methods:
The retrospective study included 132 participants from March 2020 to November 2021. Participants were divided into two groups based on the inclusion criteria: normal and abnormal Krüger strict morphology, with a mean age of 40 years. Seminal analyses were conducted following the guidelines outlined in the 6th edition of the Manual for Examination and Processing of Human Semen (2021). The sperm chromatin dispersion test was used.
Results:
The results did not reveal a statistically significant difference between Krüger strict morphology and Sperm DNA Fragmentation Index correlation (p<0.05) between the normal and abnormal morphology groups (p<0.05) and in Seminal Parameters. Sperm concentration is lower when Krüger strict morphology is < 4% (abnormal) (p=0.007).
Conclusions:
In conclusion, abnormal Krüger strict morphology does not have a higher predisposition to increased sperm DNA fragmentation.
