Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In Vitro Fertilization

In Vitro Fertilization

In vitro fertilization (IVF) is a form of assisted reproductive technology where an egg is fertilized with sperm in a controlled laboratory environment before transferring the resulting embryo into the uterus. This process is designed to help individuals and couples experiencing difficulties conceiving.
The IVF process begins with ovarian stimulation, during which reproductive endocrinologists prescribe hormonal medications to stimulate the ovaries to produce multiple eggs instead of the single...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Annual Variation in Stallion Testicular Measurements and Its Association With Hormonal Profiles and Semen Quality.

Reproduction in domestic animals = Zuchthygiene·2026

Same author

Does the Reproductive Technique Affect Neonatal Health Parameters in Foals?

Reproduction in domestic animals = Zuchthygiene·2026

Same author

Use of thermography in the long-term evaluation of scrotal surface temperature and its impact on seminal quality in stallions.

Reproduction in domestic animals = Zuchthygiene·2024

Same author

Comparative Assessment of Oxidative and Antioxidant Parameters in Mule and Horse Neonates during Their First Month of Extrauterine Adaptation.

Animals : an open access journal from MDPI·2023

Same author

Postnatal Dynamics of Circulating Steroid Hormones in Mule and Equine Neonates.

Veterinary sciences·2022

Same author

Effects of Refrigeration at 5°C for Long Periods of Time on Bovine Ear Skin as a Strategy to Transport Biological Material and Isolate Fibroblasts to Use in the Nuclear Transfer.

Biopreservation and biobanking·2021

Search research articles

Related Experiment Video

Updated: Jun 5, 2025

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Equine ICSI: an update on semen perspective.

Rodrigo Arruda de Oliveira¹, Maria Augusta Alonso², Juliana Schleich Fonte²

¹Laboratório de Reprodução Animal, Faculdade de Agronomia e Medicina Veterinária, Universidade de Brasília, Brasília, DF, Brasil.

Animal Reproduction

|December 4, 2024

Summary

This summary is machine-generated.

Intracytoplasmic Sperm Injection (ICSI) extends the use of limited stallion semen for fertility treatments. Optimizing sperm selection is crucial for successful fertilization and embryo development in equine reproduction.

Keywords:

cut straw fertilization frozen semen stallion swim up

More Related Videos

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

Related Experiment Videos

Last Updated: Jun 5, 2025

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Published on: May 11, 2017

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

U-Shaped Horizontal Swimming Technique for Preparing High-Quality Sperm with Low DNA Fragmentation Index

Published on: March 28, 2025

Area of Science:

Veterinary Reproduction
Animal Biotechnology
Equine Science

Background:

Intracytoplasmic Sperm Injection (ICSI) is increasingly vital for equine reproduction, especially with aged stallions or limited frozen semen.
Challenges include reduced sperm numbers/quality and extending the viability of cryopreserved samples.

Purpose of the Study:

To review the application and efficiency of ICSI in stallions.
To explore semen handling techniques and sperm selection methods for improved outcomes.
To discuss stallion-specific effects and future prospects of ICSI in equine breeding.

Main Methods:

Review of current literature on ICSI in stallions.
Analysis of semen processing and cryopreservation techniques.
Evaluation of various sperm selection methods (e.g., swim-up, density gradient, microfluidics).

Main Results:

ICSI enables the use of minimal spermatozoa, extending the utility of valuable or limited semen.
Sperm quality significantly impacts fertilization and embryo development, despite the possibility of success with lower quality samples.
Various selection techniques can improve the chances of successful fertilization and development.

Conclusions:

ICSI is a valuable tool for overcoming stallion fertility challenges and maximizing the use of limited semen resources.
Careful semen handling and selection of superior spermatozoa are essential for optimizing ICSI efficiency in horses.
Further research into stallion effects and technological advancements will enhance ICSI's role in equine breeding.