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Updated: Jun 5, 2025
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Multiparametric Tumor Organoid Drug Screening Using Widefield Live-Cell Imaging for Bulk and Single-Organoid Analysis
Published on: December 23, 2022
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An integrated microfluidic device for sorting of tumor organoids using image recognition
Xingyang Yan1, Deng Tan1, Lei Yu2
1Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China. chhkwu@ust.hk.
Lab on a Chip
|December 4, 2024
Summary
A novel microfluidic device automates tumor organoid sorting using image processing and microvalves. This technology enhances drug screening by enabling efficient, label-free classification and collection of diverse organoids.
Area of Science:
- Biomedical Engineering
- Cancer Research
- Microfluidics
Background:
- Tumor organoids are valuable for drug screening but exhibit heterogeneity, complicating their use.
- Current methods for organoid analysis lack efficiency and automation.
- Standardized organoid processing is crucial for reliable drug discovery.
Purpose of the Study:
- To develop a portable microfluidic system for automated tumor organoid sorting.
- To enable label-free classification and collection of organoids based on morphology.
- To improve the efficiency and reliability of organoid-based drug screening.
Main Methods:
- A portable microfluidic device integrating image processing algorithms and microvalve-controlled deflection.
- Automated organoid recognition and sorting based on morphological features.
- Experiments conducted with polystyrene beads, F9 tumoroids, and patient-derived tumor organoids.
Main Results:
- Achieved organoid separation efficiency exceeding 88%.
- Demonstrated sorting purity surpassing 91% and organoid viability exceeding 97%.
- Reached a classification throughput of 800 organoids per hour.
Conclusions:
- The morphology-activated organoid sorting system offers automated, portable, and cost-effective organoid handling.
- This technology addresses the heterogeneity challenge in tumor organoids for drug screening.
- The system meets the demands of clinical organoid medicine for efficient sample processing.
