Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

IR Spectroscopy: Molecular Vibration Overview01:24

IR Spectroscopy: Molecular Vibration Overview

1.9K
When Infrared (IR) radiation passes through a covalently bonded molecule, the bonds transition from lower to higher vibrational levels. The fundamental vibrational motions that result in infrared absorption can be classified as stretching or bending vibrations.
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
1.9K
IR and UV–Vis Spectroscopy of Aldehydes and Ketones01:29

IR and UV–Vis Spectroscopy of Aldehydes and Ketones

5.2K
Infrared spectroscopy, also known as vibrational spectroscopy, is mainly used to determine the types of bonds and functional groups in molecules. In aldehydes and ketones, the carbonyl (C=O) bond shows an absorption around 1710 cm-1. The C=O bond vibration of an aldehyde occurs at lower frequencies than that of a ketone. In addition to the C=O absorption in an aldehyde, the aldehydic C–H bond also gives two peaks in the 2700–2800 cm-1 range. This absorption, coupled with the...
5.2K
IR Spectrum Peak Broadening: Hydrogen Bonding01:23

IR Spectrum Peak Broadening: Hydrogen Bonding

840
The vibrational frequency of a bond is directly proportional to its bond strength. As a result, stronger bonds vibrate at higher frequencies, while weaker bonds vibrate at lower frequencies. The stretching vibration of the strong O–H bond in alcohols and phenols (very dilute solution or gas phase) appears as a sharp peak at 3600–3650 cm−1.
However, the extent of hydrogen bonding influences the observed stretching frequency and band broadening. Intermolecular or intramolecular...
840
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

1.4K
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
1.4K
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

1.2K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
1.2K
IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

913
Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single...
913

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering Cytochromes for Photocatalysis: Biohybrid Assemblies for Light-Driven Dye Decoloration.

New biotechnology·2026
Same author

Impact of Morphology and Composition of Graphene Aerosol-Gel Particles in Thin Films on Ultrafast Carrier Dynamics Studied via Transient Absorption Spectroscopy.

The journal of physical chemistry. C, Nanomaterials and interfaces·2026
Same author

Practical and divergent electrochemical access to thiocarbamoyl fluorides and N-trifluoromethyl amines from secondary amines.

Nature communications·2026
Same author

Erratum: "Thermal and chemical control of emission and excited-state dynamics in non-(TMS)3P-derived InP quantum dots" [J. Chem. Phys. 164, 144702 (2026)].

The Journal of chemical physics·2026
Same author

<i>In vivo</i> 2D-IR spectroscopy of [NiFe] hydrogenases: a shielding role of the protein matrix.

Physical chemistry chemical physics : PCCP·2026
Same author

Ultrafast Energy Transfer in Orthogonal Heptamethine Cyanine-Naphthalimide Systems: A Pathway toward High-Energy Excitation.

The journal of physical chemistry. A·2026
Same journal

Scanning Tunneling Microscope-Based Break-Junction TechniqueA Tutorial.

ACS physical chemistry Au·2026
Same journal

Role of Small Membrane Proteins in the Green Sulfur Bacterial Reaction Center.

ACS physical chemistry Au·2026
Same journal

The Seasons of a Career in Physical Chemistry: Olivia Harper Wilkins.

ACS physical chemistry Au·2026
Same journal

Heavy Water Remodels the DNA Energy Landscape to Stabilize Folded States and Slow Transitions.

ACS physical chemistry Au·2026
Same journal

Free-Energy Profiles of Confined Reactions: Influence of Confinement Type and Challenges for Metadynamics Methods.

ACS physical chemistry Au·2026
Same journal

Chirality Transfer in Gold Nanoclusters: Insights from Chiral Spectroscopy and Theoretical Modeling.

ACS physical chemistry Au·2026
See all related articles

Related Experiment Video

Updated: Jun 5, 2025

F&#246;rster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
07:09

Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features

Published on: March 16, 2022

2.4K

Two-Dimensional Infrared Spectroscopy Resolves the Vibrational Landscape in Donor-Bridge-Acceptor Complexes with

James D Shipp1, Ricardo J Fernández-Terán1,2, Alexander J Auty1

  • 1Department of Chemistry, University of Sheffield. Sheffield S3 7HF, U.K.

ACS Physical Chemistry Au
|December 5, 2024
PubMed
Summary
This summary is machine-generated.

Donor-bridge-acceptor complexes exhibit surprising intramolecular vibrational energy redistribution across a platinum bridge. Site-selective isotopic labeling reveals distance-dependent energy transfer and coherent states in these light-induced systems.

More Related Videos

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

17.9K
Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid
08:54

Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid

Published on: January 25, 2020

5.6K

Related Experiment Videos

Last Updated: Jun 5, 2025

F&#246;rster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features
07:09

Förster Resonance Energy Transfer Mapping: A New Methodology to Elucidate Global Structural Features

Published on: March 16, 2022

2.4K
Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy
10:03

Proton Transfer and Protein Conformation Dynamics in Photosensitive Proteins by Time-resolved Step-scan Fourier-transform Infrared Spectroscopy

Published on: June 27, 2014

17.9K
Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid
08:54

Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid

Published on: January 25, 2020

5.6K

Area of Science:

  • * Physical Chemistry
  • * Spectroscopy
  • * Materials Science

Background:

  • * Donor-bridge-acceptor (D-B-A) complexes are crucial for studying light-induced processes.
  • * Understanding vibrational energy redistribution (IVR) and transfer (VET) is key to controlling these processes.
  • * Platinum-containing acetylide bridges are common in D-B-A systems, but their role as vibrational bottlenecks is debated.

Purpose of the Study:

  • * To investigate the mechanism of vibrational energy redistribution in D-B-A complexes with a trans-Pt(II) acetylide bridge.
  • * To determine the site-specific rates of vibrational energy transfer and spectral diffusion.
  • * To elucidate the role of the heavy atom bridge in vibrational energy propagation.

Main Methods:

  • * Application of two-color two-dimensional infrared (2D-IR) spectroscopy.
  • * Site-selective 13C isotopic labeling of the acetylide bridge to decouple vibrational modes.
  • * Analysis of vibrational energy transfer rates, dynamic anharmonicities, and spectral diffusion.

Main Results:

  • * Intramolecular IVR occurs between acetylide groups even when decoupled across the platinum bridge.
  • * Vibrational energy transfer from the bridge to the acceptor is site-specific and distance-dependent.
  • * Excitation transfers to ligand-centered modes (subpicosecond) then to solvent modes (picoseconds).
  • * Isotopic substitution does not alter spectral diffusion rates.
  • * Acceptor carbonyl modes form a coherent superposition of states post-excitation.

Conclusions:

  • * The platinum acetylide bridge does not act as a complete vibrational bottleneck, allowing IVR.
  • * Isotopic labeling is a powerful tool for dissecting vibrational energy pathways in complex D-B-A systems.
  • * Findings provide insights into the vibrational dynamics governing IR-mediated electron transfer.