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Updated: Jun 5, 2025
06:04
Murine Dermal Fibroblast Isolation by FACS
Published on: January 7, 2016
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Fibroblast diversification is an embryonic process dependent on muscle contraction.
Lavi Coren1, Shelly Zaffryar-Eilot1, Anas Odeh1
1Department of Genetics and Developmental Biology, The Rappaport Faculty of Medicine and Research Institute, Technion - Israel Institute of Technology, Haifa 31096, Israel.
Cell Reports
|December 5, 2024
Summary
Skeletal muscle fibroblasts diversify into distinct types during embryonic development. This diversification, driven by muscle contraction, influences muscle growth and development.
Area of Science:
- Developmental Biology
- Cell Biology
- Tissue Engineering
Background:
- Fibroblasts are crucial for tissue function, but their diversity and roles remain incompletely understood.
- Specific fibroblast subpopulations are linked to various biological processes, yet mechanisms of their variation are not fully elucidated.
Purpose of the Study:
- To investigate the diversification of skeletal muscle-resident fibroblasts (mrFibroblasts) during embryonic development.
- To understand the distinct cellular dynamics, transcriptomes, and functional roles of mrFibroblast subpopulations.
Main Methods:
- Analysis of mrFibroblast heterogeneity during embryonic and fetal myogenesis.
- Characterization of distinct fibroblast subpopulations based on niche, cellular dynamics, and transcriptomic profiles.
- Investigation of the role of muscle contraction in fibroblast diversification.
Main Results:
- mrFibroblasts diversify into two main subpopulations: interstitial and delineating fibroblasts.
- These subpopulations exhibit distinct cellular dynamics and transcriptomes.
- mrFibroblast subpopulations differentially regulate myoblast proliferation and differentiation.
- Fibroblast diversification is dependent on muscle contraction.
Conclusions:
- mrFibroblasts diversify into distinct cell types during embryonic development.
- These distinct fibroblast subpopulations possess unique characteristics and functional roles in skeletal muscle.
- Muscle contraction is a key driver of fibroblast diversification during myogenesis.
Keywords:
CP: Developmental biologycell diversificationembryonic developmentfibroblast subpopulationsfibroblastsmsenchymal cellsmusclemuscle connective tissuemyogenesisMore Related Videos
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