Protocol for immunofluorescence staining and large-scale analysis to quantify microglial cell morphology at
Frida Lind-Holm Mogensen1, Corrado Ameli2, Alexander Skupin3
1Neuro-Immunology Group, Department of Cancer Research, Luxembourg Institute of Health, 6A, Rue Nicolas-Ernest Barblé, L-1210 Luxembourg, Luxembourg; Faculty of Science, Technology and Medicine, University of Luxembourg, 2, avenue de l'Université, L-4365 Esch-sur-Alzette, Luxembourg.
STAR Protocols
|December 5, 2024
Summary
This study details a protocol for quantifying microglial cell morphology in mouse brains at the single-cell level. This method aids in understanding microglial function in disease and therapeutic responses.
Area of Science:
- Neuroscience
- Immunology
- Cell Biology
Background:
- Microglial cell morphology is crucial for understanding their activation states.
- Assessing microglial morphology is vital in disease progression and therapeutic evaluations.
Purpose of the Study:
- To present a detailed protocol for quantifying microglial cell morphology.
- To enable single-cell level analysis of microglial morphology in mouse brains.
Main Methods:
- Protocol covers mouse brain dissection and computational analysis.
- Enables analysis of up to 350 microglial cells per brain slice.
Main Results:
- A standardized protocol for microglial morphology quantification is established.
- The protocol facilitates detailed analysis from dissection to computational methods.
Conclusions:
- This protocol provides a comprehensive method for microglial morphology analysis.
- It supports research into microglial function in various conditions and treatments.
