Protocol for immunofluorescence staining and large-scale analysis to quantify microglial cell morphology at
Frida Lind-Holm Mogensen1, Corrado Ameli2, Alexander Skupin3
1Neuro-Immunology Group, Department of Cancer Research, Luxembourg Institute of Health, 6A, Rue Nicolas-Ernest Barblé, L-1210 Luxembourg, Luxembourg; Faculty of Science, Technology and Medicine, University of Luxembourg, 2, avenue de l'Université, L-4365 Esch-sur-Alzette, Luxembourg.
Abstract:
Here, we present a protocol for quantifying microglial cell morphology at the single-cell level in mice. We provide comprehensive details, starting from optimal mouse brain dissection to computational analyses of up to 350 microglial cells per brain slice. Analyzing the morphology of microglial cells is essential for understanding their functional and activation states in different conditions, including during disease development and progression, as well as for assessing the effect of therapeutic interventions. For complete details on the use and execution of this protocol, please refer to Lind-Holm Mogensen et al.1 and Fixemer et al.2.
