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Updated: Aug 12, 2026
13:47
Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
Published on: February 24, 2015
Click-code-seq protocol for genome-wide single-nucleotide-resolution mapping of major types of DNA modifications
Lucie Lefevre1, Emilie A Aghajani1, Navnit K Singh1
1Department of Health Sciences and Technology, ETH Zurich, 8093 Zurich, Switzerland.
STAR Protocols
|August 10, 2026
Summary
This study introduces click-code-seq, a novel sequencing method for mapping DNA modifications like oxidized guanines and abasic sites across the genome at single-nucleotide resolution.
Area of Science:
- Molecular Biology
- Genomics
- Biochemistry
Background:
- DNA modifications play crucial roles in various biological processes and diseases, including cancer, aging, and neurodegeneration.
- Accurate mapping of DNA modifications is essential for understanding their functional impact.
Purpose of the Study:
- To present a detailed protocol for click-code-seq, a next-generation sequencing technique.
- To enable genome-wide, single-nucleotide-resolution mapping of major DNA modifications.
Main Methods:
- Genomic DNA extraction and library preparation.
- Enzymatic modification conversion and click-chemistry-based tagging.
- Data analysis using the Click-pipe computational pipeline.
Main Results:
- The protocol allows for precise mapping of oxidized guanines and abasic sites.
- Click-code-seq provides genome-wide coverage at single-nucleotide resolution.
Conclusions:
- Click-code-seq is a powerful new tool for studying DNA modifications.
- This technique facilitates research into the roles of DNA modifications in health and disease.
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