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Updated: Jun 4, 2026

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PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
Published on: July 2, 2010
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Crystallographic analysis of G-clamp-RNA complex assisted by large scale RNA-binding profile
Ryosuke Nagasawa1,2, Kazumitsu Onizuka1,2,3, Karen Kawamura4
1Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Miyagi 980-8577, Japan. onizuka@tohoku.ac.jp.
Abstract:
We present the X-ray crystal structure of a complex between a G-clamp and an internal loop motif of pre-mir-125a, selected from high affinity RNAs identified in a large-scale RNA-binding profile. This X-ray crystal structure reveals that the G-clamp interacts with three distinct guanine bases, forming robust bonds through hydrogen bonding and stacking interactions.

