Development of immobilized peroxidase on amino-functionalized magnetic MgFe2O4 nanoparticles for antioxidant activity
Keziban Atacan1, Nuray Güy2, Alican Bahadir Semerci3
1Sakarya University of Applied Sciences, Faculty of Technology, Department of Engineering Fundamental Sciences, 54050 Sakarya, Türkiye.
Abstract:
This investigation aims to immobilize peroxidase onto 3-aminopropyltriethoxysilane (APTES)-functionalized MgFe2O4 magnetic nanoparticles to increase enzyme stability, efficiency, and recyclability. The synthesized samples were analyzed using X-ray diffraction, Fourier transform infrared spectroscopy, Thermogravimetric analysis, Vibrating sample magnetometer, and Scanning electron microscopy. The free and immobilized peroxidase were examined against different pH and temperatures as well as storage time and reuse. The immobilized peroxidase maintained 52 % of its initial activity after 10 consecutive measurements thanks to easy magnetic separation. In addition, antioxidant activity was increased by immobilizing the peroxidase to the MgFe2O4 magnetic nanoparticles. Congo red dye removal for peroxidase immobilized MgFe2O4-APTES was found to be 98.6 % for 240 min. Also, it showed approximately two times more dye decolorization efficiency compared to MgFe2O4 and APTES modified MgFe2O4. Finally, the immobilized peroxidase was studied by a decolorization study of congo red. So, we believe that the immobilized peroxidase on magnetic nanoparticles reported in this study may be utilized in diverse biotechnology, industrial, and environmental practices.
