Impact of input volume on red cell quality in deglycerolized RBCs using a modified ACP-215 protocol

Area of Science:

• Blood banking and transfusion medicine
• Cell processing technology
• Red blood cell preservation

Background:

• The ACP 215 automated cell processor is used for glycerolizing and deglycerolizing red cell concentrates (RCCs).
• It offers a closed system for extended post-thaw expiry compared to the COBE 2991.
• Lower post-deglycerolization hematocrits (Hct) were observed with the LN236 kit.

Purpose of the Study:

• To determine if a modified process using the smaller volume LN235 deglycerolization kit can increase final Hct.
• To assess potential deleterious effects on product characteristics with the LN235 kit.

Main Methods:

• Two proof-of-concept studies were conducted to establish feasibility and identify process modifications.
• A two-part study characterized the modified protocol.
• Investigated the impact of pre-cryopreservation storage duration, input red cell mass, and RCC production method.

Main Results:

• Using the LN235 kit with volume reduction for RCCs >180 mL red cell mass increased Hct by ~8%.
• Slightly lower recoveries were observed for large RCCs due to volume reduction.
• No other detrimental outcomes on product quality were noted.

Conclusions:

• The LN235 kit can be leveraged to increase post-deglycerolization Hct for RCCs exceeding 180 mL red cell mass.
• This approach is recommended for units with higher red cell mass than the kit's standard recommendation.