Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Common structural genes for platelet and plasma fibrinogen.

B Teige, G Gogstad, F Brosstad

|January 1, 1985

Summary

Plasma and platelet fibrinogen share identical structural genes for their B beta- and gamma-chains, as confirmed by two-dimensional electrophoresis. This finding clarifies the molecular basis of fibrinogen heterogeneity in different blood components.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Platelet activation in patients with peripheral vascular disease: reproducibility and comparability of platelet markers.

Thrombosis research·2011

Same author

Glycoprotein IIb-IIIa on platelet-derived microparticles, and microparticle structures studied by electron microscopy, confocal laser microscopy and crossed radio-immunoelectrophoresis.

Platelets·2010

Same author

Fatal poisoning in drug addicts in the Nordic countries in 2007.

Forensic science international·2010

Same author

Syndecan-1 plasma levels during coronary artery bypass surgery with and without cardiopulmonary bypass.

Perfusion·2008

Same author

New biomarkers in an acute model of live Escherichia coli-induced sepsis in pigs.

Scandinavian journal of immunology·2008

Same author

No case of COX-1-related aspirin resistance found in 289 patients with symptoms of stable CHD remitted for coronary angiography.

Scandinavian journal of clinical and laboratory investigation·2007

Area of Science:

Biochemistry
Molecular Biology
Hematology

Background:

Fibrinogen, a key protein in blood coagulation, exists in both plasma and platelet forms.
Understanding the structural relationship between plasma and platelet fibrinogen is crucial for elucidating their distinct roles and potential disease associations.
Previous studies suggested potential differences in fibrinogen composition between plasma and platelets.

Purpose of the Study:

To compare the protein composition of plasma fibrinogen and platelet-derived fibrinogen.
To investigate the structural identity of the B beta- and gamma-chains of fibrinogen from plasma and platelet sources.
To determine if the structural genes encoding plasma and platelet fibrinogen chains are identical.

Main Methods:

Two-dimensional electrophoresis was employed to analyze fibrinogen isolated from human plasma and platelets.
Platelet fibrinogen was obtained from alpha-granules and thrombin/collagen-released platelet material.
Analysis included examination of A alpha-, B beta-, and gamma-chains, with a focus on genetic variants in heterozygous individuals.

Main Results:

Two-dimensional electrophoresis revealed similar patterns for the B beta- and gamma-chains of both plasma and platelet fibrinogen.
Gamma'-chains were not detected in platelet fibrinogen, and A alpha-chains were difficult to identify.
Genetic variants in B beta- and gamma-chains were consistently observed in both plasma and platelet fibrinogen from heterozygous individuals, indicating identical structural genes.

Conclusions:

The structural genes for the B beta- and gamma-chains of plasma and platelet fibrinogen are identical.
Observed differences in platelet fibrinogen (e.g., absence of gamma'-chains, difficulty identifying A alpha-chains) likely stem from post-translational modifications or differential processing rather than distinct genes.
This study provides strong evidence for a common genetic origin of fibrinogen B beta- and gamma-chains in plasma and platelets.

Related Experiment Videos