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High-throughput antibody screening with high-quality factor nanophotonics and bioprinting
Sajjad Abdollahramezani1, Darrell Omo-Lamai1, Gerlof Bosman2
1Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
Arxiv
|December 9, 2024
Summary
High-throughput nanophotonics- and bioprinter-enabled screening (HT-NaBS) rapidly characterizes antibody libraries. This label-free platform accelerates biotherapeutic discovery by efficiently screening for high-affinity antibodies against diverse targets.
Area of Science:
- Biotechnology and Nanophotonics
- Immunology and Drug Discovery
Background:
- Investigating large-scale antibody repertoires is crucial for identifying biotherapeutic leads but remains a significant challenge.
- Existing methods for antibody library characterization are often burdensome and time-consuming.
Purpose of the Study:
- To develop a high-throughput, sample-efficient, and rapid assay for characterizing antibody libraries.
- To enable the identification of potential biotherapeutic leads through advanced screening.
Main Methods:
- Utilized a multiplexed assay (HT-NaBS) employing pixelated nanoantennas with high-quality factors and high pattern densities.
- Integrated a custom acoustic bioprinter for precise, high-speed sensor functionalization with picoliter droplets.
- Employed spatially-resolved spectral imaging for simultaneous detection of antigen-antibody binding kinetics, affinity, and specificity.
Main Results:
- Demonstrated sub-picomolar limit of detection for antibodies targeting SARS-CoV-2, Influenza A, and Influenza B within 30 minutes.
- Successfully performed epitope binning analysis on antibody libraries against H5N1 and Cetuximab targets.
- Achieved rapid characterization of antibody binding kinetics, affinity, and specificity.
Conclusions:
- The HT-NaBS platform offers a high-throughput, resource-efficient, and label-free solution for antibody library screening.
- This technology accelerates the discovery of high-affinity antibodies and facilitates de novo protein design.
- Future development aims to scale imaging to tens of thousands of sensors for even broader applications.
