Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Fermentation01:23

Microbial Fermentation

Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
Lipid Catabolism01:25

Lipid Catabolism

1
Triglycerides serve as crucial long-term energy storage molecules in microorganisms, providing a dense source of metabolic energy. Their breakdown is mediated by lipases, which hydrolyze triglycerides into glycerol and free fatty acids. Each of these components follows distinct metabolic pathways, ultimately contributing to ATP synthesis and cellular energy homeostasis.Glycerol MetabolismGlycerol, released from triglyceride hydrolysis, is phosphorylated by glycerol kinase to form...
1

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydrogen-Deuterium Back-Exchange for High-Resolution Mass-Spectrometry-Based Metabolite Characterization.

Analytical chemistry·2026
Same author

Autoencoder/RandomForest-TabPFN for cross-cancer metabolomics: prostate and breast cancer diagnosis using paper spray and ion mobility-mass spectrometry techniques.

GigaScience·2026
Same author

Rapid Noninvasive Classification of Prostatic Disease Using Paper Spray Ionization Mass Spectrometry (PSI-MS)-Based Nontargeted Metabolomics.

Analytical chemistry·2026
Same author

CerS2 Is a Druggable Target in Triple-Negative Breast Cancer.

Molecular cancer therapeutics·2026
Same author

Increased <i>S. epidermidis</i> in the airway-gut microbiome of infants with bronchopulmonary dysplasia.

bioRxiv : the preprint server for biology·2026
Same author

Serum metabolomic profiles associated with psychoneurological symptoms in women with early-stage breast cancer over one year.

Frontiers in oncology·2026

Related Experiment Video

Updated: Jun 5, 2025

Author Spotlight: Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016
11:04

Author Spotlight: Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

Published on: June 23, 2023

3.3K

Erucic acid utilization by Lactobacillus johnsonii N6.2.

Sharon C Thompson1, Reagan Beliakoff1, Timothy J Garrett2

  • 1Department of Microbiology and Cell Science, Genetics Institute, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL, United States.

Frontiers in Microbiology
|December 10, 2024
PubMed
Summary
This summary is machine-generated.

Lactic acid bacteria (LAB) efficiently utilize erucic acid (EA) from rapeseed and mustard oil. This study reveals how *Lactobacillus johnsonii* N6.2 incorporates EA, synthesizing beneficial omega-9 fatty acids.

Keywords:
Lactobacillus johnsoniierucic acidlong chain fatty acidnervonic acidprobiotic

More Related Videos

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
10:41

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli

Published on: January 13, 2013

18.5K
Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
10:23

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Published on: August 23, 2024

676

Related Experiment Videos

Last Updated: Jun 5, 2025

Author Spotlight: Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016
11:04

Author Spotlight: Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

Published on: June 23, 2023

3.3K
The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli
10:41

The Logic, Experimental Steps, and Potential of Heterologous Natural Product Biosynthesis Featuring the Complex Antibiotic Erythromycin A Produced Through E. coli

Published on: January 13, 2013

18.5K
Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
10:23

Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System

Published on: August 23, 2024

676

Area of Science:

  • Microbiology
  • Nutritional Science
  • Molecular Biology

Background:

  • Erucic acid (EA), a fatty acid (FA) abundant in rapeseed and mustard oil, is linked to increased lactic acid bacteria (LAB) counts.
  • Lactobacillus johnsonii N6.2 and other LAB species can effectively metabolize EA as a primary FA source.

Purpose of the Study:

  • To investigate the metabolic pathways and genetic regulation of EA utilization by *L. johnsonii*.
  • To characterize the changes in FA profiles of *L. johnsonii* when grown with EA.
  • To identify genes and pathways involved in EA assimilation and lipid synthesis.

Main Methods:

  • Culturing *L. johnsonii* N6.2 with EA as the sole FA source.
  • Global transcriptomics to analyze gene expression changes.
  • Liquid chromatography-mass spectrometry (LC-MS) to determine FA profiles.

Main Results:

  • EA assimilation in *L. johnsonii* involves the FakA/FakB and plsX/plsY/plsC pathways for phosphatidic acid synthesis.
  • Upregulation of *fakB2*, *fakB4*, *plsY2*, *plsC2*, and *plsC4* genes observed in EA-grown cells.
  • Rapid incorporation of EA and synthesis of omega-9 monounsaturated fatty acids, including nervonic and gondoic acids, were confirmed.

Conclusions:

  • *L. johnsonii* possesses specific mechanisms for efficient EA uptake and metabolism.
  • EA serves as a precursor for synthesizing valuable long-chain fatty acids with potential health benefits.
  • This research elucidates the molecular basis of LAB adaptation to dietary fatty acids.