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Characterization of a Salmonella abortus equi phage 4FS1 and its depolymerase
Jianchao Zhao1, Jiayu Wang1, Can Zhang1
1College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, China.
Frontiers in Veterinary Science
|December 10, 2024
Summary
A novel phage, 4FS1, effectively targets the bacterium *S. abortus equi*, a significant threat in donkey husbandry. Its depolymerase enzyme shows promise as a therapeutic agent to combat infections and reduce economic losses.
Area of Science:
- Veterinary Microbiology
- Bacteriophage Therapy
- Genomics
Background:
- Economic losses in donkey husbandry due to *S. abortus equi* necessitate novel control strategies.
- Bacteriophages and their enzymes offer a potential alternative to traditional treatments.
Purpose of the Study:
- To isolate and characterize a bacteriophage targeting *S. abortus equi*.
- To evaluate the therapeutic potential of the phage and its depolymerase enzyme.
Main Methods:
- Isolation and characterization of *S. abortus equi* phage 4FS1 using transmission electron microscopy.
- Host range, stability, and genomic analysis of the phage.
- Recombinant expression and in vitro/in vivo testing of the depolymerase enzyme (Dpo36).
Main Results:
- Phage 4FS1 demonstrated optimal lytic activity and stability under various environmental conditions.
- Genomic analysis revealed no virulence or drug resistance genes.
- The depolymerase Dpo36 significantly inhibited biofilm formation and bacterial growth, enhancing survival rates in a mouse model.
Conclusions:
- Phage 4FS1 is a promising candidate for controlling *S. abortus equi* infections.
- The depolymerase Dpo36 exhibits significant therapeutic potential as a standalone agent or in combination therapy.
