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Updated: Jun 5, 2025

Testing the Role of Multicopy Plasmids in the Evolution of Antibiotic Resistance
Published on: May 2, 2018
Polymorphisms in the type A blaZ gene as determinants of the cefazolin inoculum effect in Staphylococcus aureus
Shinwon Lee1, Sohee Park1, Jeong Nam Kim2
1Department of Internal Medicine, Pusan National University School of Medicine and Medical Research Institute, Pusan National University Hospital, Busan, Republic of Korea.
Abstract:
Plasmids containing the wild-type (WT) or mutant type A blaZ (blaZA) gene (mutations at codons 226, 229, or both 226 and 229) were constructed and transformed into Staphylococcus aureus RN4220, yielding the strains WT-blaZ, M226-blaZ, M229-blaZ, and MB-blaZ. The high-inoculum cefazolin MIC was significantly lower in MB-blaZ and M226-blaZ than in WT-blaZ but not in M229-blaZ, suggesting that the single nucleotide polymorphism at codon 226 in blaZA contributes to the cefazolin inoculum effect.
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