Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

3.3K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
3.3K
Viral Structure00:56

Viral Structure

61.7K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
61.7K
Protein Complex Assembly02:41

Protein Complex Assembly

10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.6K
Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

2.8K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
2.8K
What are Viruses?00:50

What are Viruses?

113.8K
Overview
113.8K
Viruses of Archaea01:29

Viruses of Archaea

Archaeal viruses play a crucial role in the ecosystems of extremophilic archaea, particularly those belonging to the phyla Euryarchaeota and Crenarchaeota. By shaping host evolution and facilitating gene transfer, these viruses influence microbial communities and contribute to genetic diversity in extreme environments. The archaea they infect thrive in acidic hot springs and hydrothermal vents characterized by high temperatures and low pH. Archaeal viruses exhibit remarkable structural...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vitro antiproliferative activity on A549 and HT-29 cell lines and fatty acid profiling of Agaricus bresadolanus and A. hortensis.

Die Naturwissenschaften·2026
Same author

Personalized Damage Assessment in Aesthetic Surgery: Current Trends and the Italian Scenario.

Healthcare (Basel, Switzerland)·2025
Same author

Imidazole Derivative Cyclotriphosphazene-Based PLA/PEG Composite Films: Antibacterial Properties against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>.

ACS omega·2025
Same author

Proximity labelling of internalizing influenza A viruses reveals a role for neogenin in virus uptake.

PLoS pathogens·2025
Same author

Probing living cell dynamics and molecular interactions using atomic force microscopy.

Biophysical reviews·2025
Same author

Probing SARS-CoV-2 membrane binding peptide via single-molecule AFM-based force spectroscopy.

Nature communications·2025

Related Experiment Video

Updated: Jun 5, 2025

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging
05:31

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging

Published on: January 2, 2014

9.6K

From viral assembly to host interaction: AFM's contributions to virology.

Ankita Ray1, Joshua D Simpson1, Irem Demir1

  • 1Louvain Institute of Biomolecular Science and Technology, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.

Journal of Virology
|December 10, 2024
PubMed
Summary

Atomic Force Microscopy (AFM) offers unparalleled nanoscale insights into virus biology, revealing complex host-virus interactions and life cycles at the single-virus level. This versatile technique provides unique data crucial for advancing virology research and biomedical technologies.

Keywords:
atomic force microscopyentry receptorsforce spectroscopynanoindentationsingle-molecule biophysicsvirus

More Related Videos

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
09:47

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

Published on: March 1, 2012

12.2K
Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
08:30

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

22.5K

Related Experiment Videos

Last Updated: Jun 5, 2025

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging
05:31

Sample Preparation for Single Virion Atomic Force Microscopy and Super-resolution Fluorescence Imaging

Published on: January 2, 2014

9.6K
Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly
09:47

Simple and Robust in vivo and in vitro Approach for Studying Virus Assembly

Published on: March 1, 2012

12.2K
Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy
08:30

Visualization of Recombinant DNA and Protein Complexes Using Atomic Force Microscopy

Published on: July 18, 2011

22.5K

Area of Science:

  • Virology and Nanotechnology
  • Biophysics and Structural Biology

Background:

  • Viruses are obligate parasites with diverse, complex biology requiring detailed characterization.
  • Understanding virus-host interactions is vital for public health, medicine, agriculture, and ecology.

Purpose of the Study:

  • To highlight the utility and versatility of Atomic Force Microscopy (AFM) in virus research.
  • To demonstrate how AFM provides unique insights into virus biology at the single-virus level.

Main Methods:

  • Utilizing AFM's nanoscale spatial resolution for high-detail imaging.
  • Employing diverse AFM chemistries and imaging modes to explore virus-host interactions.
  • Analyzing single-virus behaviors including binding kinetics, self-assembly, and budding.

Main Results:

  • AFM enables exploration of virus structural features and life cycles at the molecular level.
  • The technique reveals complex host-virus interactions and dynamics.
  • AFM provides data unobtainable by other imaging modalities.

Conclusions:

  • AFM is a powerful and versatile tool for detailed virological studies.
  • Its ability to probe single-virus phenomena offers unique advantages.
  • Continued evolution of AFM technology will further enhance its role in virology research.