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Surface-Enhanced Raman Scattering Nanoendoscope for Quantification of a Protein Released under Physiological
Maryam Hojjat Jodaylami1,2, Ohini Yanis Sanvi2,3, Ravi L Rungta2,3,4
1Département de Chimie, Institut Courtois, Quebec Center for Advanced Materials, Regroupement Québécois sur les Matériaux de Pointe, Université de Montréal, C.P. 6128 Succ. Centre-ville, Montréal H3C 3J7, Québec, Canada.
A novel nanoendoscope uses surface-enhanced Raman scattering (SERS) to detect S100β protein in brain tissue. This SERS nanoendoscope enables precise, localized monitoring of protein changes relevant to neurological diseases.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Neuroscience
Background:
- S100β protein levels change in various neuropathologies.
- Intratissular S100β levels have not been locally monitored.
- Minimal invasiveness and high spatial resolution are needed for tissue analysis.
Purpose of the Study:
- To develop a surface-enhanced Raman scattering (SERS) nanoendoscope for detecting S100β protein.
- To demonstrate quantitative, regiospecific detection of S100β in brain tissue.
- To monitor S100β concentration changes under physiological stimuli.
Main Methods:
- Fabrication of a SERS nanoendoscope using gold nanoparticles on optical fibers.
- Modification of nanoparticles with anti-S100β antibodies for specific detection.
- Ex vivo quantitative analysis of S100β in mouse brain slices using SERS-active nanotags.
Main Results:
- The SERS nanoendoscope achieved micrometer resolution for S100β detection.
- Detection limits were 5 nM in solution and 7 nM in brain slices.
- Stimulation (NMDA, electrical, optogenetic) significantly increased S100β concentrations (27–48 nM).
Conclusions:
- The SERS nanoendoscope enables noninvasive, localized quantification of S100β in biological tissues.
- This technology can monitor protein dynamics in neurological conditions.
- The SERS nanoendoscope demonstrates potential for studying protein release under physiological conditions.
