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Labeling DNA Probes

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Updated: Jun 5, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

Label-free multiplexed detection based on core-shell photonic barcodes integrated RCA.

Dagan Zhang¹, Nan Zhang¹, Junqi Zhao¹

¹Department of Clinical Laboratory, Institute of Translational Medicine, The Affiliated Drum Tower Hospital of Nanjing University Medical School, 210008, Nanjing, China.

Biosensors & Bioelectronics

|December 10, 2024

Summary

This study introduces a novel biosensor for rapid, label-free virus quantification. The photonic crystal barcode and rolling circle amplification system can simultaneously detect multiple viruses like SARS-CoV-2 and H1N1 with high sensitivity.

Keywords:

Barcode Core-shell Label-free Photonic crystal RCA

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Last Updated: Jun 5, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Area of Science:

Nanotechnology
Biomedical Engineering
Molecular Diagnostics

Background:

Accurate and rapid virus quantification is crucial for biomedical detection.
Existing methods may lack multiplexing capabilities or sensitivity for simultaneous detection of multiple viral targets.

Purpose of the Study:

To develop a label-free, multiplexed virus screening quantitative biosensor.
To integrate photonic crystal (PhC) barcodes with rolling circle amplification (RCA) for enhanced detection.

Main Methods:

Fabrication of a core-shell hydrogel photonic crystal (PhC) barcode using acrylic acid and polyethylene glycol diacrylate.
Utilizing a silica photonic crystal core for detection and adjusting its structure for distinct color barcodes.
Implementing a DNA-decorated antibody complex with a blocked primer and RCA strategy to convert protein signals to nucleic acid signals.

Main Results:

Simultaneous detection of SARS-CoV-2-N, SARS-CoV-2-S, and H1N1 in a single reaction.
Achieved highly sensitive multiplexed quantitative detection with a detection limit as low as 0.30 pg/mL.
Validated the platform with clinical samples, demonstrating high accuracy and specificity.

Conclusions:

The developed biosensor offers a promising platform for rapid, sensitive, and accurate multiplexed virus detection.
The integration of PhC barcodes and RCA provides a versatile approach for biomedical screening.
The biosensor shows significant potential for clinical diagnosis and screening applications.