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Updated: Jun 25, 2026
Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
An enzyme-free immunosorbent assay of staphylococcal enterotoxin B using a three-way DNA junction amplifier with an
Wanhao Li1, Jiali Xing2, Jinzhong Xi3
1State Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, P.R. China. guoyahui@jiangnan.edu.cn.
Abstract:
A highly sensitive immunoadsorption assay without traditional horseradish peroxidase for signal amplification was developed, utilizing a three-way DNA junction amplifier instead. The formation of a three-way DNA junction and release of trigger DNA with recycling was accomplished by toehold-mediated strand displacement. The fluorescent dye N-methyl mesoporphyrin IX, with highly structure-specific binding affinity towards G-quadruplexes, was employed for label-free and simple signal output. High sensitivity was achieved for concentrations as low as 7.21 pg mL-1 by targeting Staphylococcal enterotoxin B for detection, and its applicability was confirmed by a recovery study of precooked dishes.
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