Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

6.6K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.6K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.5K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chitosan nanogels for the improvement of benznidazole activity.

International journal of biological macromolecules·2026
Same author

Membrane Vesicles from Lacticaseibacillus Casei BL23 Exhibit Antimicrobial Activity Against Escherichia coli and Immunostimulatory Effects on Human Peripheral Blood Mononuclear Cells.

Advanced healthcare materials·2025
Same author

Perspectives on the pH-Influenced Design of Chitosan-Genipin Nanogels for Cell-Targeted Delivery.

Pharmaceutics·2025
Same author

Progress in the design of chitosan-based nanogels for folic acid delivery for ocular applications.

International journal of biological macromolecules·2025
Same author

Chitosan-TPP Nanogels for Ocular Delivery of Folic Acid: Release Profile, Corneal Permeation, and Mucoadhesion Assessment.

Pharmaceutics·2025
Same author

Bovine Serum Albumin Nanoparticle-Mediated Delivery of Ribavirin and Mycophenolic Acid for Enhanced Antiviral Therapeutics.

Viruses·2025

Related Experiment Video

Updated: Jun 5, 2025

Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas
10:35

Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas

Published on: February 10, 2018

33.5K

Quinoa Proteins Isolate, a Candidate for Functional Ingredients Design.

Alejandra J Rubinstein1, Oscar E Pérez2

  • 1Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Consejo Nacional de Investigación Científica y Técnicas de la República Argentina, IQUIBICEN-CONICET, Universidad de Buenos Aires, Intendente Güiraldes, s/n, Ciudad Universitaria, Buenos Aires, CP 1428, Argentina.

Plant Foods for Human Nutrition (Dordrecht, Netherlands)
|December 11, 2024
PubMed
Summary

Quinoa protein nanocomplexes loaded with resveratrol and tocopherol exhibit enhanced antioxidant properties. These functional ingredients demonstrate potential for use in food and nutraceutical applications.

Keywords:
ComplexationIsolatesQuinoa ProteinsResveratrolTocopherol

More Related Videos

Designing Silk-silk Protein Alloy Materials for Biomedical Applications
11:14

Designing Silk-silk Protein Alloy Materials for Biomedical Applications

Published on: August 13, 2014

18.3K
Author Spotlight: Enhancing Flavor Interaction of Food Using Matcha Microcapsules
05:22

Author Spotlight: Enhancing Flavor Interaction of Food Using Matcha Microcapsules

Published on: July 26, 2024

631

Related Experiment Videos

Last Updated: Jun 5, 2025

Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas
10:35

Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas

Published on: February 10, 2018

33.5K
Designing Silk-silk Protein Alloy Materials for Biomedical Applications
11:14

Designing Silk-silk Protein Alloy Materials for Biomedical Applications

Published on: August 13, 2014

18.3K
Author Spotlight: Enhancing Flavor Interaction of Food Using Matcha Microcapsules
05:22

Author Spotlight: Enhancing Flavor Interaction of Food Using Matcha Microcapsules

Published on: July 26, 2024

631

Area of Science:

  • Food Science
  • Biochemistry
  • Materials Science

Background:

  • Quinoa protein (QP) is a valuable source of plant-based protein with potential for developing functional ingredients.
  • Resveratrol (RSV) and tocopherol (TOC) are potent antioxidants with health benefits, but their bioavailability can be limited.
  • Encapsulation strategies are needed to improve the stability and efficacy of bioactive compounds like RSV and TOC.

Purpose of the Study:

  • To design and characterize resveratrol (RSV) and tocopherol (TOC) loaded quinoa protein (QP) nanocomplexes.
  • To evaluate the antioxidant and antiradical functionality of these novel nanocomplexes.
  • To understand the interaction mechanisms between QP and the bioactive compounds.

Main Methods:

  • Quinoa protein (QP) nanocomplexes loaded with resveratrol (RSV) and tocopherol (TOC) were designed and characterized.
  • Intrinsic fluorescence spectroscopy was used to study protein-bioactive compound interactions.
  • Stern-Volmer and Scatchard models were applied to analyze quenching effects.
  • Isothermal titration calorimetry (ITC) provided thermodynamic parameters of interaction.
  • Atomic force microscopy (AFM) was used to detect protein aggregate formation.

Main Results:

  • Fluorescence quenching indicated interactions between QP and both RSV and TOC, consistent with static quenching.
  • Complexation parameters derived from Stern-Volmer and Scatchard models quantified the binding affinity.
  • ITC revealed thermodynamic signatures of the interaction between QP and the bioactive compounds.
  • AFM confirmed the formation of QP aggregates, up to 40 nm in diameter, induced by RSV and TOC.
  • QP-RSV and QP-TOC nanocomplexes exhibited an additive effect on antioxidant capacity.

Conclusions:

  • Quinoa protein can effectively complex with resveratrol and tocopherol, forming stable nanocomplexes.
  • These nanocomplexes demonstrate significant antioxidant and antiradical activity, potentially enhanced by additive effects.
  • The developed QP-RSV and QP-TOC nanocomplexes represent a promising platform for creating functional food ingredients with improved bioactive compound delivery.