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Updated: Jun 5, 2025
Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
Published on: October 27, 2017
Protocol for feeding strategy and proteomics analysis of zebrafish Danio rerio using S-trap and iTRAQ techniques
Kathiresan Purushothaman1, Daanish Kaul2, Saraphina Dianne Tneo Rwei Qing3
1Department of Preclinical Sciences and Pathology, Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, Norway; Tropical Futures Institute, James Cook University Singapore, 149 Sims Drive, Singapore 387380, Singapore; School of Applied Science, Republic Polytechnic, 9 Woodlands Avenue 9, Singapore 738964, Singapore.
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Yeast serves as a functional alternative and sustainable protein source in aquaculture. This protocol outlines feeding strategy and intestinal proteome analysis of zebrafish (Danio rerio), using S-trap for digestion, iTRAQ, and mass spectrometry for protein quantification. Additionally, it details the analysis of chemical components in feed and functional assessments via Kyoto Encyclopedia of Genes and Genomes (KEGG), Eukaryotic Orthologous Group (KOG), and Gene Ontology (GO). For complete details on the use and execution of this protocol, please refer to Purushothaman et al.1.
