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Updated: Jun 5, 2025
In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids
Published on: November 18, 2022
High internal-phase Pickering emulsions constructed using myofibrillar proteins from large yellow croaker: Effect of
Min Lin1, Yufeng Chen2, Linfan Shi3
1College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China.
Abstract:
Exploring the emulsification of myofibrillar protein (MP) from large yellow croaker (Larimichthys crocea) could meet the demand for high-value development and utilization of fish proteins. Therefore, MPs as the emulsifier to form stable high internal-phase Pickering emulsions (HIPPEs) with the addition of glycerol and the effects of different glycerol addition ratios of HIPPEs were investigated. HIPPEs could be constructed by MPs with the glycerol addition at a ratio of 15 %-30 % (v/v) compared to those without the addition of glycerol. With the increase in glycerol ratios, the absolute value of Zeta potential increased and reached 22.57 ± 0.29 mV at the glycerol ratio of 30 %. All the HIPPEs stabilized by MPs with the addition of glycerol possessed storage stability. Besides, the centrifugal stability constant (Ke), backscattered light intensity and reflected light stability index confirmed that the addition of glycerol was beneficial for the formation of stable HIPPEs prepared by MPs. Additionally, HIPPEs stabilized by MPs with the addition of glycerol possessed small emulsion droplets and viscoelastic behavior. These findings could be helpful for the development and utilization of MPs of large yellow croaker in the food industry.
