3D Printed GelMA/CHIMA Cross-Linked Network Hydrogel for Angiogenesis

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Regenerative Medicine

Background:

• Vascularization is crucial for the success of 3D tissue constructs in transplantation.
• Existing biomaterials often lack the necessary properties for promoting vascularization.

Purpose of the Study:

• To develop a novel bioink for 3D printing bioactive scaffolds with angiogenesis-inducing capabilities.
• To enhance the mechanical and biological properties of hydrogels for tissue engineering applications.

Main Methods:

• Preparation of a photocurable hydrogel bioink by combining carboxymethyl chitosan (CHIMA) and methacryloyl gelatin (GelMA).
• Utilizing 3D printing technology to fabricate bioactive scaffolds from the GelMA/CHIMA bioink.
• Evaluating the structural, mechanical, and biological properties of the scaffolds, including cell viability and angiogenesis gene expression.

Main Results:

• The cross-linked GelMA/CHIMA bioink exhibited a porous structure supporting cell growth and metabolism.
• CHIMA incorporation significantly improved the hydrophilicity, swelling, pressure resistance, and mechanical strength of the bioink.
• The bioink supported the survival and proliferation of human umbilical vein endothelial cells (HUVECs).
• A specific formulation (8 wt% GelMA/2 wt% CHIMA) stimulated angiogenesis gene expression.
• 3D printed scaffolds demonstrated HUVEC survival and deposition of angiogenesis-related proteins (CD31, VEGF).

Conclusions:

• A bioactive scaffold with angiogenesis induction function was successfully constructed using GelMA/CHIMA bioink.
• This provides a promising strategy for developing vascularized complex tissues.
• The developed bioink offers enhanced properties for tissue engineering and regenerative medicine applications.