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Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments
Published on: February 3, 2023
[Experimental substantiation of medical device design for mechanical processing of adipose tissue]
I I Eremin1, V S Vasiliev2, A N Tikhomirov3
1Petrovsky National Research Center of Surgery, Moscow, Russia.
Objective:
To substantiate the medical device design for mechanical processing of adipose tissue.
Material And Methods:
Enzymatic method using collagenase and mechanical method for obtaining the stromal vascular fraction (SVF) are compared. The authors emphasized the disadvantages of enzymatic method, such as high cost and duration of process. The main criteria for developing the design were cell viability after processing of adipose tissue and available processing in a minimum number of passages manually without automated mechanical stands.
Results:
The proposed design allows two unidirectional passages to obtain processed lipoaspirate for SVF harvesting. Experimental studies demonstrate that meshes with a minimum cell size of 100 μm allow high cell viability comparable to enzymatic method. Moreover, this design can be used to develop a disposable medical device.
Conclusion:
Our results will underlie development of a disposable medical device for mechanical processing of adipose tissue.
