Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

4.6K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
4.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

7.5K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
7.5K
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

2
Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
2
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

2
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
2
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.0K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ultrafast and steady-state optical characterization of multilayer PdS<sub>2</sub>.

Nanoscale·2026
Same author

Building a cost-effective mechanochemical Raman system: improved spectral and time resolution for <i>in situ</i> reaction and rheology monitoring.

Physical chemistry chemical physics : PCCP·2025
Same author

Green and Scalable Preparation of Highly Conductive Alkali Metal-dhta Coordination Polymers.

Inorganic chemistry·2024
Same author

Disulfide-Containing Nitrosoarenes: Synthesis and Insights into Their Self-Polymerization on a Gold Surface.

Langmuir : the ACS journal of surfaces and colloids·2024
Same author

Application of the Knife-Edge Technique on Transition Metal Dichalcogenide Monolayers for Resolution Assessment of Nonlinear Microscopy Modalities.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2024
Same author

Short-Term l-arginine Treatment Mitigates Early Damage of Dermal Collagen Induced by Diabetes.

Bioengineering (Basel, Switzerland)·2024
Same journal

Deep Learning Based Framework for Detection and Classification of Leukemia Using Microscopic Images.

Microscopy research and technique·2026
Same journal

Externally Controlled In Situ SEM: Multi-Rate Scanning With Signal Regulation and Spatiotemporal Fusion.

Microscopy research and technique·2026
Same journal

In Situ TEM Observation of Phase Transformation Nucleation at the Near-Surface of Synthetic Aragonite.

Microscopy research and technique·2026
Same journal

Morpho-Anatomical and HPTLC Investigations of Lysimachia nummularia L. (Primulaceae) Grown in Switzerland.

Microscopy research and technique·2026
Same journal

Macroscopic, Histological and Ultrastructural Features of the Tongue of the Anatolian Wild Boar (Sus scrofa libycus).

Microscopy research and technique·2026
Same journal

Ultrastructural Insights Into the Reproductive Anatomy and Eggs of Cotton Pink Bollworm, Pectinophora gossypiella Saunders (Lepidoptera: Gelechiidae).

Microscopy research and technique·2026
See all related articles

Related Experiment Video

Updated: Jun 5, 2025

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.2K

Development of Structured Illumination Microscope Using Transmission Diffraction Grating Obtained by Analog

Aleksa Denčevski1, Jovana Z Jelić1, Ana Senkić2

  • 1Institute of Physics Belgrade, University of Belgrade, National Institute of the Republic of Serbia, Belgrade, Serbia.

Microscopy Research and Technique
|December 13, 2024
PubMed
Summary
This summary is machine-generated.

We developed a custom structured illumination microscope (SIM) using a novel diffraction grating fabrication method. This advanced microscope achieves enhanced resolution, revealing fine cellular structures like vimentin filaments in astrocytes.

Keywords:
diffraction gratingresolution measurementstructured illumination microscopysuper‐resolution microscopy

More Related Videos

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.7K
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

6.9K

Related Experiment Videos

Last Updated: Jun 5, 2025

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

12.2K
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

9.7K
High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
14:09

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

Published on: November 16, 2019

6.9K

Area of Science:

  • Optical Microscopy
  • Nanotechnology
  • Biophysics

Background:

  • Structured Illumination Microscopy (SIM) offers super-resolution capabilities.
  • Diffraction gratings are crucial optical components in microscopy.
  • Developing cost-effective and customizable fabrication methods for optical components is essential.

Purpose of the Study:

  • To develop a custom-built structured illumination microscope (SIM).
  • To fabricate custom transmission diffraction gratings using an analog microfilming method.
  • To evaluate the resolution enhancement of the developed SIM system.

Main Methods:

  • Fabrication of transmission diffraction gratings via analog microfilming.
  • Resolution assessment using fluorescent beads and the knife-edge technique on MoS2 monolayers.
  • Imaging of fluorescently labeled astrocytes, focusing on vimentin filaments.

Main Results:

  • Successful fabrication of robust, cost-effective diffraction gratings with customized constants.
  • Demonstrated resolution enhancement of SIM over epifluorescent imaging using two independent methods.
  • Acquired super-resolution images revealing fine vimentin cytoskeleton structures in astrocytes.

Conclusions:

  • The custom-built SIM system with bespoke diffraction gratings achieves enhanced resolution.
  • The analog microfilming method provides a versatile approach for fabricating optical components.
  • The developed SIM effectively visualizes fine subcellular structures not visible with conventional microscopy.