Electron microscopic studies on Blastocrithidia triatomae Cerisola et al. 1971 (Trypanosomatidae)

In the bug Triatoma infestans developmental stages of Blastocrithidia tritomae, which can be mistaken for T. cruzi during xenodiagnosis, were studied by means of electron microscopy. It turned out that three different forms appeared during the progressive divisions: "micro-", "pro-" and epimastigotes. The morphological study revealed significant criteria for a distinction from T. cruzi stages. Furthermore cyst-like bodies were observed often attached to the flagella of the different stages. As revealed by the presence of labyrinthine structures found in flagellated stages and in the cyst stages the latter were suggested as being specific cysts to be excreted with the feces and thus to establish new infections in other bugs. Apart from the cyst-like structures a comparison with the organelles known from other Trypanosomatidae showed only slight variations. A tubular cytopharynx-like structure, which was lined by microtubules, and the way of attachment of the flagellum to the surface of the host cell has not been described in Blastocrithidia before.