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Updated: Jun 5, 2025

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

A Rapid High-throughput Method for Mapping Ribonucleoproteins RNPs on Human pre-mRNA

Published on: December 2, 2009

Functional RNA mining using random high-throughput screening.

Li-Hua Liu¹, Jinde Chen¹, Shijing Lai¹

¹Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd., Tongchaunghui South District, No. 40, Shangchong South, Haizhu District, Guangzhou, Guangdong 510000, P.R. China.

Nucleic Acids Research

|December 14, 2024

Summary

This summary is machine-generated.

A new random high-throughput screening (rHTS) method efficiently identifies functional RNA molecules. This technology discovered novel RNA fragments impacting bacterial growth and coenzyme conversion, showcasing its power for RNA mining.

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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Functional RNA molecules play critical roles in cellular processes.
Current methods for screening functional RNA are limited.
Developing novel screening technologies is essential for advancing RNA research.

Purpose of the Study:

To develop and validate a novel random high-throughput screening (rHTS) technology for identifying functional RNA.
To apply rHTS for discovering RNA molecules involved in bacterial growth and coenzyme metabolism.

Main Methods:

Synthesis of a large, uniform library of random RNA fragments (~250-nt).
Circularization of RNA fragments using an auto-cyclizing ribozyme for enhanced stability.
Application of rHTS to screen for RNA affecting Escherichia coli growth.
Utilizing rHTS on a coenzyme-dependent platform to identify RNA modulating NADPH/NADP+ conversion.

Main Results:

Identified three RNA fragments significantly promoting Escherichia coli growth, one with coding potential.
Discovered two noncoding RNAs (ncRNAs) that inhibit E. coli growth in vivo.
Identified two ncRNAs that promote the conversion of NADPH to NADP+.
Demonstrated increased glycerol dehydrogenase activity by 18.3% to 21.8% and 23.2% with exogenous expression of identified ncRNAs.

Conclusions:

The developed random high-throughput screening (rHTS) technology is a powerful tool for functional RNA discovery.
rHTS enables the identification of novel ncRNAs with significant biological functions.
This technology has broad applications in various fields, including synthetic biology and metabolic engineering.