Microbial collagenases: an updated review on their characterization, degradation mechanisms, and current applications

Collagen, recognized as a fundamental protein present in biological tissues and structures, plays a crucial role in maintaining organ structure and tissue integrity. Microbial collagenases are specific for the degradation of collagen. The specific three-stranded helix region of natural collagen can be identified and hydrolyzed by microbial collagenases under physiological conditions, producing collagen peptides with high physiological activity. This article describes microbial collagenases, providing an introduction to the structure, physiological characteristics, factors affecting enzyme activity, and hydrolysis mechanisms of various classes of these enzymes. Microbial collagenase is the most widely used class of collagenase and plays an important role in all aspects of human life, and various applications of microbial collagenases in food industry, healthcare and environmental protection will be addressed in this review. In addition to its beneficial functions, microbial collagenase can exist as a virulence factor for pathogenic bacteria, and enhanced research on its structure and mechanism of action will help us to investigate more effective inhibitors as well as therapeutic agents and tools for the treatment of the corresponding diseases. Finally, this review critically analyses existing challenges and outlines prospects for future advancements in the field.