Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Imperfections in Crystal Structure: Stoichiometric Point Defects

Imperfections in Crystal Structure: Stoichiometric Point Defects

Schottky defects arise when some lattice points in a crystal, such as those in NaCl, remain unoccupied, creating lattice vacancies without disturbing the overall electrical neutrality of the crystal. This defect is common in ionic crystals where the positive and negative ions are similar in size, as seen in sodium chloride and cesium chloride. The presence of Schottky defects enables the crystal to conduct electricity to a small extent through an ionic mechanism. Electric fields cause nearby...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Advances in antioxidant activities of edible mushroom polysaccharides: status, influencing factors, mechanisms, and applications.

Frontiers in nutrition·2026

Same author

Complement Factor H as a Novel Predictive Biomarker for Deep Vein Thrombosis Recurrence: A Prospective Cohort Study.

Journal of thrombosis and thrombolysis·2026

Same author

Knowledge, Attitude, Practice, and Awareness Regarding Disease Management Among Patients with Chronic Lower Limb Ischemia: A Cross-Sectional Study.

Patient preference and adherence·2026

Same author

Hypoxic mesenchymal stem cell-Derived Exosomal circPTP4A2 improves granulosa cell mitochondrial function via YBX1.

Cellular and molecular life sciences : CMLS·2026

Same author

Immune-molecular nexus in reproductive disorders: mechanisms linking POI and RSA.

Frontiers in genetics·2025

Same author

LncRNA RWDD3 Facilitates Leydig Cell Steroidogenesis by Regulating the miR-1388-5p/NPY1R/cAMP Pathway in Yanshan Cashmere Goats.

Animals : an open access journal from MDPI·2025

Same journal

Protocol for semi-automatic quantitative bioimaging analysis of synapse loss.

STAR protocols·2026

Same journal

Protocol for integrated ubiquitination analysis of in vitro E3 ligase-DUB regulation and in vivo ubiquitin chain linkage characterization.

STAR protocols·2026

Same journal

Protocol for constructing multi-ancestry polygenic models using S4-Multi.

STAR protocols·2026

Same journal

Protocol for inducing deep vein thrombosis in C57BL/6J mice using the inferior vena cava stenosis model.

STAR protocols·2026

Same journal

Overcoming the challenges of genome-editing essential genes.

STAR protocols·2026

Same journal

Protocol for longitudinal two-photon calcium imaging and holographic optogenetic manipulation to investigate memory in mice.

STAR protocols·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 12, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Protocol to study secretome interactions using extracellular proximity labeling.

Joshua A Rich¹, Sadeechya Gurung¹, Sasha Coates-Park¹

¹Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA.

|December 14, 2024

Summary

This summary is machine-generated.

This study introduces a new method for identifying protein interactions outside of cells using proximity ligation. This protocol helps researchers understand the extracellular interactome of secreted proteins.

Keywords:

Biotechnology and bioengineering Cell Biology Mass Spectrometry Proteomics

More Related Videos

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

Related Experiment Videos

Last Updated: Jun 12, 2026

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions

Published on: November 23, 2015

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Extracellular Protein Microarray Technology for High Throughput Detection of Low Affinity Receptor-Ligand Interactions

Published on: January 7, 2019

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

Area of Science:

Biochemistry
Molecular Biology
Proteomics

Background:

Biotin ligase proximity ligation is effective for studying in vivo protein-protein interactions.
Limited methods exist for studying proximal interactomes of secreted factors.
Consensus on optimal methods for extracellular interactome studies is lacking.

Purpose of the Study:

To present an adapted proximity ligation protocol for studying extracellular proximal interactomes.
To provide detailed steps for cell preparation, sample collection, and processing.
To outline procedures for biotinylated protein enrichment, digestion, and pull-down.

Main Methods:

Adaptation of TurboID/BioID2-based proximity ligation for extracellular studies.
Detailed protocol for cell preparation and sample collection.
Procedures for on-bead digestion and post-pull-down processing.

Main Results:

A refined protocol for investigating extracellular protein proximity interactions.
Successful application of TurboID/BioID2 for secreted factor interactome analysis.
Demonstration of key steps including enrichment, digestion, and processing.

Conclusions:

The presented protocol offers a robust method for studying extracellular proximal interactomes.
This adaptation of proximity ligation expands its utility to secreted factors.
The protocol facilitates a deeper understanding of extracellular protein interactions.