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Updated: Jun 5, 2025
Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
Published on: November 13, 2021
Determination of parathion by time-resolved fluorescence immunochromatographic assay based on nanobody: Aiming at
Jiarui Pang1, Mingwei Guo1, Yu Wang2
1Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Abstract:
The label probe plays a crucial role in enhancing the sensitivity of immunochromatographic assay. Time-resolved fluorescent microspheres (TRFMs) have provided accurate and reliable results for fast and immediate detection. In this study, parathion was used as a model molecule in the time-resolved fluorescence immunochromatographic assay (TRFICA) strip establishment using recognition element of anti-parathion nanobody (VHH9). Under the optimal conditions, this TRFICA demonstrated good analytical performance for the detection of parathion with a cut-off value of 30 ng/mL and the limit of detection (LOD) of 0.016 ng/mL. Meanwhile, the sensitivity of the nanobody-based TRFICA was 10-fold and 480-fold higher than that of the VHH9 based colloidal gold ICA (CGICA) and commercial mAb-ICA. Moreover, this method exhibited good recoveries for the detection of cabbage, cucumber, and orange samples with no obvious cross-reactions with the other analogs observed and showed strong correlation with UPLC-MS/MS results in spiked samples. In summary, the proposed method is feasible, rapid, and could serve as a highly sensitive platform to meet practical samples detection for parathion.
