Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Communication compartments in mixed cell cultures.

J D Pitts, E Kam

    Experimental Cell Research
    |February 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Preoperative systemic inflammation and perioperative myocardial injury: prospective observational multicentre cohort study of patients undergoing non-cardiac surgery.

    British journal of anaesthesia·2019
    Same author

    Association between preoperative pulse pressure and perioperative myocardial injury: an international observational cohort study of patients undergoing non-cardiac surgery.

    British journal of anaesthesia·2017
    Same author

    Preoperative heart rate and myocardial injury after non-cardiac surgery: results of a predefined secondary analysis of the VISION study.

    British journal of anaesthesia·2016
    Same author

    Comparison of Propofol-Lipuro with propofol mixed with lidocaine 10 mg on propofol injection pain.

    Anaesthesia·2004
    Same author

    Metabolic co-operation between biochemically marked mammalian cells in tissue culture. 1969.

    Reviews in medical virology·2002
    Same author

    In vivo NADH fluorescence monitoring as an assay for cellular damage in photodynamic therapy.

    Photochemistry and photobiology·2002
    Same journal

    The AP2α/PDHA1 signaling pathway promotes lung cancer progression by mediating aerobic glycolysis and inhibiting cuproptosis.

    Experimental cell research·2026
    Same journal

    Distributed transcription activity in DLX proteins defies conventional mapping of a transcription activation domain.

    Experimental cell research·2026
    Same journal

    Extracellular release of cytotoxic aggregates from HSV-1-replicating SH-SY5Y cells: involvement of alpha-synuclein and poly-ubiquitin conjugates.

    Experimental cell research·2026
    Same journal

    MiR-1281 downregulates LMX1B to inhibit gastric cancer development.

    Experimental cell research·2026
    Same journal

    Corrigendum to "Heat shock protein 90β stabilizes focal adhesion kinase and enhances cell migration and invasion in breast cancer cells" [Exp. Cell Res., 326, 1, 1 August 2014, Pages 78-89].

    Experimental cell research·2026
    Same journal

    Neutrophilic differentiation of promyelocytic HL-60 cells is associated with increased expression of terminal galactose residues on the cell surface.

    Experimental cell research·2026
    See all related articles

    Mixed cell cultures form communication compartments where cells self-organize. Intercellular communication is restricted between these domains, suggesting cell surface features influence junction formation and compartmentalization.

    Area of Science:

    • Cell biology
    • Developmental biology
    • Biophysics

    Background:

    • Epithelial (BRL) cells and fibroblasts (BHK) co-cultures exhibit self-organization into distinct domains.
    • This self-organization creates communication compartments with restricted intercellular transfer across domain boundaries.

    Purpose of the Study:

    • To investigate the extent and mechanisms of intercellular communication within and between segregated cell domains.
    • To determine if cell sorting influences the formation and function of intercellular junctions.

    Main Methods:

    • Utilized electrical coupling, dye coupling, and metabolic coupling assays to assess intercellular communication.
    • Analyzed the movement of small ions and molecules across domain boundaries.

    Main Results:

    Related Experiment Videos

    • Demonstrated free movement of small molecules within domains but restricted movement between domains.
    • Metabolic coupling proved most sensitive for detecting trans-boundary communication.
    • Reduced intercellular transfer is attributed to fewer intercellular junctions between heterologous cells, not smaller channel diameters.

    Conclusions:

    • Cell sorting into distinct domains leads to the formation of communication compartments.
    • Cell surface features responsible for sorting also reduce heterologous junction formation, causing compartmentalization.
    • Established concentration gradients within compartments are relevant to pattern formation in developmental systems.