Related Experiment Video
Updated: Jun 5, 2025

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
Published on: May 21, 2020
Genome-wide association study and transcriptome analysis reveal natural variation of key genes regulation flowering
Xingru Xiang1,2, Ping Qiu1,2, Zhichao Mei1,2
1College of Agronomy, Hunan Agricultural University, Changsha, 410128 China.
Abstract:
Flowering time is a crucial determinant of both the yield and oil quality of rapeseed as well as a key indicator of plant maturity. We performed a genome-wide association study and transcriptome analysis to identify key genes/loci regulating flowering time in Brassica napus L. Forty-six haplotype regions harboring candidate genes were determined to be significantly associated with flowering time, and 28 of these haplotype regions overlapped with previously reported quantitative trait loci. A further investigation of these haplotype regions revealed nucleotide variations in the genes BnaFT-A02, BnaFRI-A10, and BnaFPA-A09 that correlated with phenotypic variations in flowering time. Furthermore, the co-expression network analysis indicated that BnaFT-A02 is directly linked to BnaFRI-A10 and BnaFPA-A09, in a subnetwork and also associated with 13 vernalization, 31 photoperiod, 33 autonomous pathway, and 10 gibberellin pathway genes, forming a potential network regulating flowering time in rapeseed. These results provide valuable haplotype markers for the breeding of early maturing rapeseed varieties.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s11032-024-01479-4.
More Related Videos
12:36Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo
Published on: January 14, 2016
08:33Laser-Capture Microdissection RNA-Sequencing for Spatial and Temporal Tissue-Specific Gene Expression Analysis in Plants
Published on: August 5, 2020
Related Concept Videos
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression at Multiple Steps
Transcriptional Regulation: Riboswitches