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Updated: Jun 5, 2025
08:51
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR RS-PCR
Published on: November 4, 2016
9.6K
Genome sequences of Staphylococcus aureus RN4220
1Division of Bacterial Disease Research, Center for Infectious Disease Research, National Institute of Infectious Diseases, National Institute of Health, Korea Disease Control and Prevention Agency, Osong, South Korea.
Microbiology Resource Announcements
|December 16, 2024
Summary
We present the complete genome sequence of Staphylococcus aureus RN4220, a key bacterial model. This high-quality genome data aids future research into Staphylococcus aureus.
Area of Science:
- Microbiology
- Genomics
- Molecular Biology
Background:
- Staphylococcus aureus is a significant human pathogen.
- The RN4220 strain is widely used in research for its genetic manipulability.
- A complete and accurate genome sequence is crucial for understanding its biology and pathogenesis.
Purpose of the Study:
- To generate a high-quality, complete whole genome sequence of Staphylococcus aureus RN4220.
- To provide a foundational genomic resource for the research community.
Main Methods:
- Whole genome sequencing using PacBio DNA sequencing technology.
- De novo assembly of the sequencing reads to reconstruct the genome.
Main Results:
- A single, contiguous genome sequence of 2,697,647 base pairs was obtained.
- The genome contains 2,652 predicted genes, including protein-coding sequences, tRNAs, and rRNAs.
Conclusions:
- The generated whole genome sequence of Staphylococcus aureus RN4220 is a high-quality, complete resource.
- This genomic data will facilitate further studies on Staphylococcus aureus virulence, antibiotic resistance, and genetic manipulation.
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