Association of R3HDM1 variants with growth and meat quality traits in Qinchuan cattle and its role in lipid
Miaoli Wang1, Wentao Zhang1, Chuang Li1
1College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Abstract:
The R3H domain containing 1 (R3HDM1) gene has emerged as a candidate influencing residual feed intake and beef yield. Despite this, the genetic variation of R3HDM1 and its effects on beef cattle remain unexplored. This study identified four single nucleotide polymorphisms (SNPs) in the R3HDM1 gene of Qinchuan cattle, with the g.61695680 T > C SNP significantly associated with chest depth and backfat thickness. The g.61695680 T > C synonymous mutation significantly altered the RNA secondary structure and stability of R3HDM1. RNA interference experiments demonstrated that R3HDM1 knockdown reduced adipogenesis and lipid accumulation in bovine preadipocytes by modulating key adipogenic factors such as CEBPβ (P < 0.05), ACCα (P < 0.05), and ATGL (P < 0.01). These findings suggest that the g.61695680 T > C variants within R3HDM1 could serve as valuable molecular markers for selecting improved Qinchuan cattle, thus enhancing genetic selection strategies for beef production.
More Related Videos
10:59Label-Free Imaging of Lipid Storage Dynamics in Caenorhabditis elegans using Stimulated Raman Scattering Microscopy
Published on: May 28, 2021
09:09Protocol for Assessing the Relative Effects of Environment and Genetics on Antler and Body Growth for a Long-lived Cervid
Published on: August 8, 2017