Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 4, 2025

Cytotoxicity Assays with Zebrafish Cell Lines
08:22

Cytotoxicity Assays with Zebrafish Cell Lines

Published on: January 6, 2023

6.2K

Standardized Protocol for Resazurin-Based Viability Assays on A549 Cell Line for Improving Cytotoxicity Data

Jessica Petiti1, Sabrina Caria1,2, Laura Revel1

  • 1Division of Advanced Materials Metrology and Life Sciences, Istituto Nazionale di Ricerca Metrologica (INRIM), 10135 Turin, Italy.

Cells
|December 17, 2024
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fibrin Gel as a Versatile Biomaterial Platform in the Biomedical Landscape: Chemical, Physical, and Biological Insights.

Gels (Basel, Switzerland)·2026
Same author

Clonal Dynamics of FLT3-ITD from Diagnosis to Relapse: Ultra-Sensitive Patient-Specific Monitoring by ddPCR.

International journal of molecular sciences·2026
Same author

New Candidate Reference Measurement Procedures for MET CNV Detection and Quantification Using Digital PCR.

Biological procedures online·2026
Same author

Implementing an integrated molecular classification for gastric cancer from endoscopic biopsies using on-slide tests.

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie·2024
Same author

Standard Operating Procedure to Optimize Resazurin-Based Viability Assays.

Biosensors·2024
Same author

Comprehensive Molecular Profiling of NPM1-Mutated Acute Myeloid Leukemia Using RNAseq Approach.

International journal of molecular sciences·2024
Same journal

RETRACTED: Bakshi et al. Crocin Inhibits Angiogenesis and Metastasis in Colon Cancer via TNF-α/NF-kB/VEGF Pathways. <i>Cells</i> 2022, <i>11</i>, 1502.

Cells·2026
Same journal

Correction: Verde et al. Molecular Mechanisms of Protein Aggregation in ALS-FTD: Focus on TDP-43 and Cellular Protective Responses. <i>Cells</i> 2025, <i>14</i>, 680.

Cells·2026
Same journal

Inflammation in Cardiomyopathies: Cellular Mechanisms Across Cardiac Phenotype.

Cells·2026
Same journal

IL-4/IL-13-Driven Dysregulation of Epidermal Lipid Metabolism in Atopic Dermatitis: An Immunometabolic Link Between Type 2 Inflammation and Barrier Dysfunction.

Cells·2026
Same journal

Activity of DNA- and RNA-Guided Prokaryotic Argonautes in Human Mitochondria.

Cells·2026
Same journal

Placental Pathophysiology in Maternal Psychoactive Substance Use: Biological, Clinical, and Forensic Perspectives.

Cells·2026
See all related articles

This study optimizes the resazurin assay for A549 cell viability testing in 2D and 3D models. Standardizing this assay improves the reliability of cytotoxicity data for drug screening.

Area of Science:

  • Biomedical Research
  • Cell Biology
  • Drug Discovery

Background:

  • The A549 cell line is crucial for cancer research and cytotoxicity studies.
  • Resazurin-based assays are widely used for cell viability assessment in drug screening.
  • Inconsistencies in pre-clinical drug screening data necessitate assay standardization.

Purpose of the Study:

  • To optimize resazurin-based viability assays for A549 cells in 2D and 3D models.
  • To enhance the reliability and reproducibility of cytotoxicity results.
  • To establish a standard operating procedure for consistent drug screening outcomes.

Main Methods:

  • Optimization of resazurin assay parameters for A549 cells.
  • Application of the assay in both 2D cell cultures and 3D fibrin gel models.
Keywords:
A549 cell linecell viability assaysconsistency of resultspre-clinical drug testsresazurin assay

More Related Videos

Imaging Cell Viability on Non-transparent Scaffolds &#8212; Using the Example of a Novel Knitted Titanium Implant
07:28

Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant

Published on: September 7, 2016

11.3K
Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

46.1K

Related Experiment Videos

Last Updated: Jun 4, 2025

Cytotoxicity Assays with Zebrafish Cell Lines
08:22

Cytotoxicity Assays with Zebrafish Cell Lines

Published on: January 6, 2023

6.2K
Imaging Cell Viability on Non-transparent Scaffolds &#8212; Using the Example of a Novel Knitted Titanium Implant
07:28

Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant

Published on: September 7, 2016

11.3K
Viability Assays for Cells in Culture
12:03

Viability Assays for Cells in Culture

Published on: January 20, 2014

46.1K
  • Focus on minimizing measurement uncertainty for improved data accuracy.

    • Main Results:

    • Successfully optimized resazurin assay protocols for A549 cells.
    • Demonstrated enhanced reliability of cytotoxicity measurements.
    • Developed a standardized procedure for consistent assay performance.

    Conclusions:

    • Standardization of the resazurin assay significantly improves the reliability of A549 cell cytotoxicity data.
    • The optimized assay and new standard operating procedure are vital for advancing pre-clinical drug screening.
    • This work provides a foundation for more robust and reproducible drug development research.