Related Experiment Video

Updated: Jun 4, 2025

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries
08:49

Identification of Mediators of T-cell Receptor Signaling via the Screening of Chemical Inhibitor Libraries

Published on: January 22, 2019

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Tryptanthrin Down-Regulates Oncostatin M by Targeting GM-CSF-Mediated PI3K-AKT-NF-κB Axis

Na-Ra Han1,2, Hi-Joon Park3, Seong-Gyu Ko2,4

  • 1College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.

Nutrients
|December 17, 2024
PubMed
Abstract

Insights

Tryptanthrin (TRYP) effectively inhibits Oncostatin M (OSM) release from neutrophils by down-regulating the PI3K-AKT-NF-κB pathway. This natural compound shows potential for treating inflammatory conditions.

Area of Science:

  • Immunology
  • Pharmacology
  • Natural Products Chemistry

Background:

  • Oncostatin M (OSM) plays a role in inflammatory responses.
  • Tryptanthrin (TRYP) is a bioactive alkaloid from indigo plants.

Purpose of the Study:

  • To investigate TRYP's inhibitory activity on OSM release from neutrophils.
  • To explore TRYP's effects on neutrophils-like differentiated (d)HL-60 cells and mouse bone marrow neutrophils.

Main Methods:

  • Stimulation of neutrophils-like dHL-60 cells and mouse neutrophils with granulocyte-macrophage colony-stimulating factor (GM-CSF).
  • Assessment of OSM production and mRNA expression levels.
  • Analysis of signaling pathway phosphorylation, including PI3K, AKT, and NF-κB.

Main Results:

  • TRYP reduced OSM production and mRNA expression in GM-CSF-stimulated neutrophils-like dHL-60 cells.
  • TRYP decreased OSM production in GM-CSF-stimulated mouse bone marrow neutrophils.
  • TRYP inhibited the phosphorylation of PI3K, AKT, and NF-κB in stimulated neutrophils-like dHL-60 cells.

Conclusions:

  • TRYP inhibits OSM release from neutrophils by down-regulating the PI3K-AKT-NF-κB signaling pathway.
  • TRYP demonstrates potential as a therapeutic agent for inflammatory diseases.
Keywords:
inflammationneutrophilsoncostatin Mtryptanthrin

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