MS SIEVE-Pushing the Limits for Biomolecular Mass Spectrometry

Area of Science:

• Analytical Chemistry
• Biochemistry
• Molecular Biology

Background:

• Electrospray mass spectrometry (ESI-MS) is crucial for
• omics
• and life sciences.
• Poor ionization efficiency and spectral contamination limit biomolecule detection in ESI-MS.

Purpose of the Study:

• To introduce MS SIEVE (MS Spectral Impurity Eliminator & Value Enhancer), an add-on device for ESI sources.
• To evaluate the efficacy of MS SIEVE in enhancing signal intensity and reducing contaminants for diverse analytes.

Main Methods:

• MS SIEVE was integrated into the electrospray source between the needle and the mass spectrometer cone.
• The device's performance was tested with proteins, peptides, lipids, glycans, DNA oligonucleotides, and synthetic polymers.

Main Results:

• MS SIEVE significantly improved signal intensity for various biomolecules.
• The device effectively suppressed spectral contributions from contaminants like NaCl.
• MS SIEVE demonstrated broad applicability across different analyte types and ESI sources.

Conclusions:

• MS SIEVE is a versatile add-on device that enhances ESI-MS performance by boosting analyte signals and reducing noise.
• This technology offers a promising solution for improving routine
• omics
• analyses and tackling challenging analytes in mass spectrometry.