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Updated: Jun 4, 2025

Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
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Oxidative Stress Monitoring Platform: A Longitudinal In vitro Multinuclear (1H/19F) MR Spectroscopic Study.

Pravat K Mandal1,2,3, Yashika Arora4, Avantika Samkaria4,5

  • 1Neuroimaging and Neurospectroscopy (NINS) Laboratory, National Brain Research Centre, Gurgaon, India. mandalpk@pitt.edu.

Cell Biochemistry and Biophysics
|December 17, 2024
PubMed
Summary
This summary is machine-generated.

Glutathione (GSH) levels decrease significantly when exposed to isoflurane, indicating oxidative stress. This study introduces a novel non-invasive MR spectroscopy method to monitor this interaction and detect residual isoflurane.

Keywords:
19F Spectroscopy1H SpectroscopyGlutathione (GSH)In-vitroIsofluraneMEGA-PRESSOxidative stress

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Area of Science:

  • Biochemistry
  • Neuroscience
  • Medical Imaging

Background:

  • Glutathione (GSH) is a crucial antioxidant depleted in neurodegenerative diseases like Alzheimer's and Parkinson's.
  • Inhaled anesthetics, such as isoflurane, have been linked to GSH depletion, unlike intravenous agents like propofol.

Purpose of the Study:

  • To evaluate the impact of isoflurane on GSH levels in an in vitro model using non-invasive magnetic resonance (MR) spectroscopy.
  • To develop a method for detecting residual isoflurane in solution concurrently with GSH monitoring.

Main Methods:

  • Utilized a 3T MR scanner with 1H and 19F coils to acquire data.
  • Employed MEGA-PRESS pulse sequence for GSH acquisition and 19F MRS for isoflurane detection.
  • Processed data using KALPANA and Siemens packages, developing a unique data processing method.

Main Results:

  • Isoflurane significantly reduced GSH levels in a phantom model compared to control (p=0.016), indicating isoflurane-induced oxidative stress.
  • Demonstrated the capability to detect residual isoflurane using 19F MR spectroscopy.
  • Reported a novel, sensitive method for simultaneous 1H and 19F MRS data generation and processing.

Conclusions:

  • The developed non-invasive MR spectroscopy approach effectively monitors GSH-isoflurane interactions and oxidative stress.
  • This methodology shows promise for clinical application in monitoring anesthetic effects and can be extended to other inhaled anesthetics.
  • Further development is needed for clinical translation of the 19F MR spectroscopy technique.