Related Experiment Video
Updated: Jun 4, 2025

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
Published on: June 4, 2020
Near-infrared fluorescent HaloTag ligands for efficient organelle labelling in live cells
Yoshiki Tanaka1, Masayasu Taki2, Shigehiro Yamaguchi1,2
1Department of Chemistry, Graduate School of Science and Integrated Research Consortium on Chemical Sciences (IRCCS), Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan. yamaguchi@chem.nagoya-u.ac.jp.
Abstract:
A series of HaloTag ligands that incorporate near-infrared (NIR)-emissive phospha-rhodamine (POR) dyes have been developed. The PORs that contain dealkylated amino groups exhibit a hypsochromic shift in their absorption and emission wavelengths. Subsequent refinement of the POR spirocyclization equilibrium significantly enhanced membrane permeability, thereby leading to a substantial reduction in the time necessary for labelling. Consequently, target organelles could even be labeled at low POR dye concentrations in the tens of nM range.
Related Concept Videos
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Protein Dynamics in Living Cells
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Tagging and Fusion Proteins

