Controlled release of trifluralin herbicide using luminescent Vibrio-derived polyhydroxyalkanoate (PHA) microcapsules

The controlled release of herbicides using new and safe materials can mitigate environmental pollution. Polyhydroxyalkanoate (PHA) is a type of biopolymer that can be produced by various bacteria. It has properties that make it suitable for encapsulation and controlled release applications. A luminescent bacterium, Vibrio sp. VLC strain was used as the PHA producer in this study. Initially, the polymer was synthesized by the bacterium following optimization of the culture medium, resulting in an approximate yield of 25 %. Subsequently, the produced polymer was analyzed using TEM, FTIR, and H-NMR techniques. Microcapsules were produced using the emulsion method. FE-SEM imaging revealed spherical microcapsules with an average diameter of 0.5-2 μm. The herbicide loading content and encapsulation efficiency were determined to be 16.64 % and 66.56 %, respectively. The herbicidal effect of the microcapsules containing trifluralin was investigated using Amaranthus retroflexus and Setaria viridis plants, demonstrating a significant reduction in various parameters after application. Furthermore, the impact of encapsulated herbicide on soil microbial population was assessed, revealing a less negative effect compared to its free form. These findings suggest that the PHA from a luminescent vibrio holds promise as an eco-friendly, biodegradable, nontoxic material for the controlled release of herbicides.