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A sensitive PnpR-based biosensor for p-nitrophenol detection.

Shuxin Wei1, Sibo Chen2, Hang Yan1

  • 1Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture and Rural Affairs, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.

International Journal of Biological Macromolecules
|December 18, 2024
PubMed
Summary
This summary is machine-generated.

A novel biosensor was developed for detecting p-nitrophenol (PNP), a common industrial pollutant. This PnpR transcription factor-based sensor offers high sensitivity and specificity for environmental monitoring.

Keywords:
BiosensorPNPPnpR

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Area of Science:

  • Environmental Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • p-nitrophenol (PNP) is a widespread industrial pollutant posing environmental risks.
  • Biosensors offer a sensitive alternative for monitoring environmental pollutants.
  • Identifying novel sensing elements is key to developing specific and sensitive detection methods.

Purpose of the Study:

  • To identify a specific sensing element for p-nitrophenol (PNP) detection.
  • To develop and optimize a PnpR transcription factor-based biosensor for PNP.
  • To evaluate the biosensor's performance in liquid and solid environmental matrices.

Main Methods:

  • Identification of the PnpR transcription factor and its interaction with the pnpC1 promoter in P. putida DLL-E4.
  • Development of a PnpR-based biosensor for PNP detection in liquid solutions.
  • Optimization using a transcriptional amplifier and construction of an agarose gel entrapment biosensor.
  • Evaluation of detection limits, linear ranges, and performance in M9 solid agar and soil samples.

Main Results:

  • The PnpR transcription factor was identified as a specific inducer for PNP.
  • The biosensor demonstrated improved sensitivity with a 100-fold lower detection limit (10 μg/L) after optimization.
  • The biosensor showed effective PNP detection in liquid (linear range 0.01-1.0 mg/L) and solid matrices (5-60 mg/L in agar, 16.8 mg/kg in soil).

Conclusions:

  • A highly sensitive and specific PnpR-based biosensor for PNP detection was successfully developed.
  • The optimized biosensor exhibits significant improvements in detection limit and output.
  • The biosensor shows potential for efficient, on-site environmental monitoring of PNP in various matrices.