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Differential Mobility Spectrometry Acoustic Ejection Mass Spectrometer System for Screening Isomerization-Mediating

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We developed DAEMS, a novel high-throughput screening method combining ion mobility and mass spectrometry. This system efficiently separates and quantifies metabolic isomers, crucial for identifying new drug targets in infectious diseases.

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Metabolomics

Background:

  • Metabolomics involves numerous structural isomers requiring advanced separation techniques.
  • Current methods struggle with high-throughput separation of isomeric metabolites.
  • Isomerase enzymes are critical in metabolism and represent potential drug targets.

Purpose of the Study:

  • To introduce and validate DAEMS (Differential Mobility Spectrometry coupled with Acoustic Ejection Mass Spectrometry) for high-throughput screening.
  • To demonstrate DAEMS's capability in separating and quantifying structural isomers in complex biological samples.
  • To showcase DAEMS as a tool for identifying drug targets by analyzing isomerase activity.

Main Methods:

  • Integration of differential mobility spectrometry (DMS) with acoustic ejection mass spectrometry (AEMS).
  • Development of ultrahigh throughput sample introduction technology.
  • Application of DAEMS for simultaneous quantitation of substrates and products of isomerase reactions.

Main Results:

  • Achieved high resolving powers (1588 and 1948) for phosphoglycerate and citrate/isocitrate isomers, surpassing previous metabolomic studies.
  • Successfully demonstrated DAEMS for separating substrates and products of iPGM, chorismate mutase, and triosephosphate isomerase.
  • Validated quantitation without internal standards due to high-speed separations compatible with AEMS.

Conclusions:

  • DAEMS offers a unique solution for high-throughput mass spectrometry requiring isomer and isobar separation.
  • The system enables efficient screening of isomerase activities for drug discovery, particularly for pathogen-specific targets.
  • DAEMS represents a significant advancement in metabolomic analysis and drug development.