Related Experiment Video
Updated: Jun 4, 2025
A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
Published on: July 13, 2018
Mitochondrial Membrane Potential and Lactate Dehydrogenase Activity in a Model of Acute In Vitro Ischemia/Reperfusion
Deepthi Ashok1, Haley Garbus2, Obialunanma V Ebenebe3
1Division of Cardiology, Department of Medicine, Johns Hopkins University, Baltimore, MD, USA.
Abstract:
Experimental models of cardiac ischemia/reperfusion injury have served as useful tools in isolating the sequence of events and mechanisms involved following an infarct. The in vitro coverslip ischemia model in neonatal myocytes is key in observing acute cellular and organelle changes during ischemia and in reperfusion. Here we use neonatal mouse ventricular myocytes, and describe two experimental readouts of lactate dehydrogenase assay, for cell damage/injury and measurement of mitochondrial membrane potential.
