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Related Experiment Videos

Charged amphiphiles regulate heart contractility and sarcolemma-Ca2+ interactions.

K D Philipson, G A Langer, T L Rich

    The American Journal of Physiology
    |January 1, 1985
    PubMed
    Summary

    Charged amphiphiles modulate calcium interactions with heart cell membranes. Anionic dodecyl sulfate enhanced Ca2+ binding and muscle contractility, while cationic dodecyltrimethylamine had opposite effects.

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    Area of Science:

    • Biochemistry
    • Cardiology
    • Cell Biology

    Background:

    • Sarcolemma plays a critical role in excitation-contraction coupling.
    • Calcium ions (Ca2+) are central to myocardial contractility.
    • Phospholipid analogues can be used to probe membrane interactions.

    Purpose of the Study:

    • To investigate the effects of charged amphiphiles on Ca2+ interaction with myocardial sarcolemma.
    • To evaluate how varying head groups of amphiphiles influence sarcolemmal function.
    • To assess the impact of amphiphiles on cardiac muscle contractility.

    Main Methods:

    • Utilized charged amphiphiles (dodecyl sulfate, dodecyltrimethylamine, lauryl acetate) as phospholipid analogues.
    • Examined Ca2+ binding and Na+-Ca2+ exchange in sarcolemmal vesicles.

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  • Measured contractility parameters (developed force, rate of force development) in rabbit papillary muscle.

    • Main Results:

    • Anionic dodecyl sulfate significantly augmented Ca2+ binding and Na+-Ca2+ exchange by ~80%.
    • Dodecyl sulfate increased rabbit papillary muscle contractility by ~100% without affecting rest tension.
    • Cationic dodecyltrimethylamine exhibited opposing effects on these parameters.
    • Neutral lauryl acetate showed minimal impact on measured variables.

    Conclusions:

    • Charged amphiphiles serve as valuable tools for studying excitation-contraction coupling.
    • Modulating sarcolemmal Ca2+ interactions with amphiphiles can alter cardiac contractility.
    • Further research using these amphiphiles can elucidate complex cellular processes in contractile tissues.