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A chromosome-scale reference genome assembly for Triplophysa lixianensis.

Chunlin He¹, Xinhui Zhang², Zhengyong Wen³

¹Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610065, China.

Scientific Data

|December 20, 2024

Summary

We generated a high-quality reference genome for the Lixian plateau loach (Triplophysa lixianensis), providing a valuable resource for its conservation and breeding. This genomic data aids evolutionary studies and understanding of this important fish species.

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Area of Science:

Genomics
Aquatic Biology

Background:

The Lixian plateau loach (Triplophysa lixianensis) is an important fish species with ecological and economic value.
Limited genomic resources have hindered in-depth evolutionary and functional studies of T. lixianensis.

Purpose of the Study:

To construct a chromosome-scale reference genome assembly for Triplophysa lixianensis.
To provide a valuable genetic resource for evolutionary studies, molecular breeding, and conservation efforts.

Main Methods:

Integration of MGI short-read, PacBio HiFi long-read, and Hi-C sequencing technologies.
Chromosome anchoring and gene annotation of the assembled genome.

Main Results:

A 668-Mb haplotypic genome assembly for T. lixianensis was successfully constructed.
98.91% of sequences were anchored to 25 chromosomes, with 23,774 protein-coding genes annotated.
The genome assembly exhibited a moderate repeat content (35.63%) and good synteny with T. dalaica.

Conclusions:

The generated genome assembly is a valuable resource for Triplophysa lixianensis.
This genomic data will facilitate evolutionary research, molecular breeding, and conservation strategies for this fish species.