Overview of chitin dissolution, hydrogel formation and its biomedical applications

Chitin hydrogel and hydrogel-based products are some of the frequently reported biomaterials for biomedical applications. Yet there is a void in understanding chitin's dissolution mechanism and its most suitable solvent system(s). Chitin is a natural polysaccharide polymer which can be dissolved in solvents such as calcium chloride- methanol, sodium hydroxide/urea (NaOH/urea), lithium chloride diacetamide (LiCl/DMAc), ionic liquids and deep eutectic solvents. Among the alkali/urea dissolution systems such as NaOH/urea, KOH/urea, LiOH/urea for dissolution of chitin we will be focussing on NaOH-based system here for ease of comparison with the other systems. Chitin has been used for decades in the biomedical field; however, new solvent systems are still being explored even to this day to identify the most suitable chemical(s) for dissolving it. Chitin, due to its biocompatibility, allows us to use it for multifaceted purposes. Hence, it is important to consolidate the available studies for better understanding about the most sought-after biomaterial. This overview deeply delves into the mechanism of action of the existing solvent systems and highlights its merits and demerits. It discusses the rheological properties of the chitin gel from different solvent systems and puts forth the current biomedical applications of chitin gel in areas such as tissue engineering, drug delivery, biosensing, hemostasis and wound healing. It also outlines recent advances and highlights the potential gaps which need to be addressed in future studies.