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Updated: Jun 4, 2025

Single-Molecule Analysis of Sf9 Purified Superprocessive Kinesin-3 Family Motors
Published on: July 27, 2022
Multicolor Tracking of Molecular Motors at Nanometer Resolution
Joseph Slivka1, Ahmet Yildiz2,3
1Department of Physics, University of California, Berkeley, CA, USA.
None:
Molecular motors move processively along cytoskeletal filaments by stepping of their motor domains (MDs). Observation of how the MDs step relative to each other reveals the mechanism of motor processivity and various gating mechanisms used by motors to coordinate the catalytic cycles of their MDs. This chapter will discuss developments in simultaneous observation of the stepping motions of the two MDs of processive motors using two-color single-particle tracking microscopy.Techniques presented: FIONA, multicolor tracking/image registration.

