An integrated wearable microfluidic biosensor for simultaneous detection of multiple biomarkers in sweat

Simultaneous detection of biomarkers in sweat is crucial for comprehensive health assessment and personalized monitoring. However, the low sweat secretion rate and low metabolite concentrations present challenges for developing non-invasive wearable sensors. This study aims to develop a flexible wearable biosensor for simultaneous detection of low-concentration biomarkers in sweat, enabling comprehensive health assessment. This study synthesized an innovative bimetallic tungstate Ag@Ag₂WO₄ and evaluated its performance for detecting uric acid (UA, 10-1000 μM), dopamine (DA, 3-500 μM), and tyrosine (Tyr, 5-1000 μM). The detection limits (LODs) for DA, UA, and Tyr sensors were 3.10 μM, 8.47 μM, and 4.17 μM, respectively, with relative standard deviations (RSDs) of 4.76 %, 2.66 %, and 3.51 %, respectively. Additionally, this study designed a hydrophilic microfluidic collection system inspired by bamboo leaf structures to enhance sweat collection efficiency. Validation studies demonstrated that the wearable biosensor effectively detects UA and TA in the sweat of volunteers. We believe this research could contribute to advancing personalized healthcare by improving the convenience and effectiveness of health monitoring technologies.