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Related Experiment Video

Updated: May 13, 2026

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids

Published on: June 5, 2020

Engineering the 3D structure of organoids.

Samuel P Moss¹, Ezgi Bakirci¹, Adam W Feinberg²

¹Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, PA, USA.

Stem Cell Reports

|December 20, 2024

Summary

Engineering approaches and biofabrication strategies enhance organoid development for improved disease modeling and regenerative medicine. These methods address limitations in current organoid models, leading to more complex and functional tissue constructs.

Keywords:

3D bioprinting biofabrication organoids spheroids stem cells vascularization

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Last Updated: May 13, 2026

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Area of Science:

Biotechnology
Stem Cell Biology
Tissue Engineering

Background:

Organoids self-organize from stem cells, creating diverse cell and tissue types.
Current organoid models face limitations in structural complexity, functional fidelity, and control over cellular microenvironments.
Difficulties in regulating cell type, arrangement, and interactions hinder the translational relevance of existing organoid systems.

Purpose of the Study:

To discuss engineering approaches for creating more complex organoids with enhanced function and translational relevance.
To explore biofabrication strategies for manipulating organoid composition, 3D organization, and scale-up.
To evaluate the advantages and disadvantages of these advanced organoid generation methods.

Main Methods:

Review of engineering strategies for organoid development.
Analysis of biofabrication techniques for controlling cellular and structural properties.
Discussion of methods to improve organoid scale-up and reproducibility.

Main Results:

Engineering and biofabrication offer precise control over organoid cellular composition and 3D architecture.
These approaches overcome limitations in self-organizing organoid models, improving tissue complexity and function.
Enhanced organoids demonstrate greater utility in disease modeling, drug screening, and regenerative medicine.

Conclusions:

Advanced engineering and biofabrication are crucial for developing sophisticated organoid models.
These improved organoid systems hold significant promise for advancing biomedical research and therapeutic applications.
Optimized organoid development facilitates more accurate disease modeling and effective drug discovery pipelines.